Exhaled breath condensate cytokine patterns in chronic obstructive pulmonary disease
Exhaled breath condensate cytokine patterns in chronic obstructive pulmonary disease
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DOI：
10.1016/j.rmed.2005.02.041
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发表时间：
2005-10-01
影响因子：
4.3
通讯作者：
Wirtz, H
中科院分区：
文献类型：
--
作者：
Gessner, C;Scheibe, R;Wirtz, H
稳定期慢性阻塞性肺疾病（COPD）、加重期COPD、无明显COPD的吸烟者和健康志愿者中细胞因子模式的差异应出于病理生理学和治疗原因而引起关注。采用灌洗法、活检法和痰液法检测肺组织中细胞因子的含量。对于系统性比较，呼出气冷凝液（EBC）似乎是非常适合的，我们调查了健康志愿者，吸烟者没有明显的COPD，稳定和恶化的COPD患者（+/-吸入类固醇），最后那些恶化的机械通气不可避免的，一个更完整的图片炎症细胞因子在COPD。我们选择EBC是因为它是非侵入性的，并且可以在自主呼吸个体和机械通气期间重复使用。EBC细胞因子（IL-1 β、IL-6、IL-8、IL-10、IL-12 p70、TNF-α）使用多重阵列检测试剂盒从单个样本中检测。稳定期COPD和志愿者在IL-1 β和IL-12方面的细胞因子模式仅表现出较小的差异（P
Differences in cytokine patterns in stable chronic obstructive pulmonary disease (COPD), exacerbated COPD, smokers without apparent COPD, and healthy volunteers should be of interest for pathophysiological and therapeutic reasons. Methods including Lavage, biopsy and sputum have been employed to investigate cytokines in the lung. For asystematic comparison, exhaled breath condensate (EBC) appears to be well suited.We investigated healthy volunteers, smokers without apparent COPD, stable and exacerbated COPD patients (+/- inhalative steroids) and finally those whose exacerbation made mechanical ventilation inevitable, for a more complete picture of inflammatory cytokines in COPD. We chose EBC because it is non-invasive and can be used repeatedly in spontaneous breathing individuals and during mechanical ventilation. EBC cytokines (IL-1 beta, IL-6, IL-8, IL-10, IL-12p70, TNF-alpha) were assayed from a single sample using a multiplex array test kit.We observed a significant increase of all cytokines in acute exacerbation compared to stable COPD, smokers, and volunteers. Stable COPD and volunteers exhibited only small differences in cytokine pattern with respect to IL-1 beta and IL-12 (P