Ultrasensitive RNA In Situ Hybridization for Detection of Restricted Clonal Expression of Low-Abundance Immunoglobulin Light Chain mRNA in B-Cell Lymphoproliferative Disorders
Ultrasensitive RNA In Situ Hybridization for Detection of Restricted Clonal Expression of Low-Abundance Immunoglobulin Light Chain mRNA in B-Cell Lymphoproliferative Disorders
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DOI：
10.1309/ajcpjtwk07fsabrj
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发表时间：
2013-11-01
影响因子：
3.5
通讯作者：
Ma, Xiao-Jun
中科院分区：
文献类型：
--
作者：
Tubbs, Raymond R.;Wang, Hongwei;Ma, Xiao-Jun
目的：目的探讨一种新的超灵敏明视野原位杂交技术（BRISH）检测B细胞非霍奇金淋巴瘤（NHL）中κ和λ免疫球蛋白信使RNA（mRNA）表达的可行性。在109例可评价病例中，108例（99%）BRISH轻链限制性（LCR）结果与FCM一致。额外的小B细胞淋巴瘤队列成功evaluated.Conclusions：BRISH分析κ和λ免疫球蛋白mRNA的表达是一个敏感的工具，建立LCR在B细胞NHL时，FCM结果不可用。
Objectives: To assess the feasibility of using a novel ultrasensitive bright-field in situ hybridization approach (BRISH) to evaluate kappa and lambda immunoglobulin messenger RNA (mRNA) expression in situ in B-cell non-Hodgkin lymphoma (NHL).Methods: A series of 110 semiconsecutive clinical cases evaluated for lymphoma with historic flow cytometric (FCM) results were assessed with BRISH.Results: BRISH light chain restriction (LCR) results were concordant with FCM in 108 (99%) of 109 evaluable cases. Additional small B-cell lymphoma cohorts were successfully evaluated.Conclusions: BRISH analysis of kappa and lambda immuno globulin mRNA expression is a sensitive tool for establishing LCR in B-cell NHL when FCM results are not available.