MEI-1/MEI-2 katanin-like microtubule severing activity is required for Caenorhabditis elegans meiosis.
MEI-1/MEI-2 katanin-like microtubule severing activity is required for Caenorhabditis elegans meiosis.
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DOI：
10.1101/gad.14.9.1072
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发表时间：
2000-05
影响因子：
10.5
通讯作者：
M. Srayko;D. Buster;O. A. Bazirgan;F. McNally;P. Mains
中科院分区：
文献类型：
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作者：
M. Srayko;D. Buster;O. A. Bazirgan;F. McNally;P. Mains
秀丽隐杆线虫减数分裂纺锤体与第一个有丝分裂纺锤体在形态上不同，但这两种结构在同一细胞质中形成约20分钟。mei-1和mei-2基因在C. elegans是卵母细胞减数分裂纺锤体的建立所必需的，但不是有丝分裂纺锤体功能所必需的。mei-1编码AAA ATP酶家族成员，其与异源二聚体海胆微管切断蛋白katanin的p60催化亚基相似。我们报告说，梅-2编码一个280个氨基酸的蛋白质含有一个类似于katanin的p80靶向亚基的区域。MEI-1和MEI-2抗体修饰减数分裂纺锤体微管和减数分裂染色质的极性末端。我们发现MEI-2的亚细胞位置取决于野生型mei-1的活性，反之亦然。这些实验，结合MEI-1和MEI-2与p60和p80 katanin的相似性，表明C. elegans蛋白质作为复合物起作用。为了支持这一观点，MEI-1和MEI-2在HeLa细胞中物理结合。此外，MEI-1和MEI-2在HeLa细胞中的共表达导致微管的解体。这些数据使我们得出结论，MEI-1/MEI-2微管切割活性是C.优美的
The Caenorhabditis elegans meiotic spindle is morphologically distinct from the first mitotic spindle, yet both structures form in the same cytoplasm approximately 20 minutes apart. The mei-1 and mei-2 genes of C. elegans are required for the establishment of the oocyte meiotic spindle but are not required for mitotic spindle function. mei-1 encodes an AAA ATPase family member with similarity to the p60 catalytic subunit of the heterodimeric sea urchin microtubule-severing protein, katanin. We report that mei-2 encodes a 280-amino acid protein containing a region similar to the p80-targeting subunit of katanin. MEI-1 and MEI-2 antibodies decorate the polar ends of meiotic spindle microtubules and meiotic chromatin. We find that the subcellular location of MEI-2 depends on wild-type mei-1 activity and vice versa. These experiments, combined with MEI-1 and MEI-2's similarity to p60 and p80 katanin, suggest that the C. elegans proteins function as a complex. In support of this idea, MEI-1 and MEI-2 physically associate in HeLa cells. Furthermore, co-expression of MEI-1 and MEI-2 in HeLa cells results in the disassembly of microtubules. These data lead us to conclude that MEI-1/MEI-2 microtubule-severing activity is required for meiotic spindle organization in C. elegans.