Molecular cloning and differential expression of somatic and testis-specific H2B histone genes during rat spermatogenesis.
Molecular cloning and differential expression of somatic and testis-specific H2B histone genes during rat spermatogenesis.
复制标题
大鼠精子发生过程中体细胞和睾丸特异性 H2B 组蛋白基因的分子克隆和差异表达。
DOI：
10.1016/0012-1606(87)90455-6
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发表时间：
1987
影响因子：
2.7
通讯作者：
Chae,CB
中科院分区：
文献类型：
--
作者：
Kim,YJ;Hwang,I;Tres,LL;Kierszenbaum,AL;Chae,CB
我们克隆了睾丸特异性组蛋白TH2B （H2B的一种变体）和大鼠体细胞H2B基因的cDNA，以研究大鼠精子发生过程中睾丸特异性组蛋白基因的调控。从DNA序列推断出的氨基酸序列显示，两组蛋白n端三分之一存在广泛的序列差异。其余的是高度保守的。在TH2B中发现1个半胱氨酸残基。除h3组蛋白外，体细胞组蛋白中不存在半胱氨酸。我们利用序列发散区作为杂交探针，研究了TH2B和H2B基因的表达。TH2B基因仅在睾丸中表达，在出生后14天检测到该基因的表达，在第20天达到最大值。H2B mRNA水平呈反比模式。随着睾丸的成熟，精原细胞和精母细胞的比例逐渐改变。原位细胞杂交研究表明，H2B基因主要表达于增殖性精原细胞和粗粒精母细胞中，而TH2B基因仅表达于粗粒精母细胞中，粗粒精母细胞在出生后14天左右首次出现在睾丸中。H2B和TH2B基因似乎是研究精子发生过程中增殖和分化事件及其调控机制的理想标记。
We have cloned cDNA of a testis-specific histone, TH2B (a variant of H2B), and rat somatic H2B gene to investigate regulation of testis-specific histone genes during rat spermatogenesis. The amino acid sequences deduced from DNA sequences show extensive sequence divergence in the N-terminal third of the two histones. The rest is highly conserved. One cysteine residue was found in TH2B. No cysteine is present in somatic histones except in H3histone. We investigated the expression of TH2B and H2B genes using the regions of sequence divergence as hybridization probes. The TH2B gene is expressed only in the testis, and the expression of this gene is detected 14 days after birth, reaching a maximum at Day 20. The level of H2B mRNA shows a reciprocal pattern. This contrasting pattern can be explained by the gradually changing proportion of spermatogonia and spermatocytes with testicular maturation.In situcytohybridization studies show that H2B gene is expressed primarily in proliferating spermatogonia and preleptotene spermatocytes, whereas TH2B gene is expressed exclusively in pachytene spermatocytes which first appear in testis about 14 days after birth. H2B and TH2B genes appear to be ideal markers for the study of proliferation and differentiation events in spermatogenesis and their regulatory mechanisms.