Structure and function of microbial communities during the early stages of revegetation of barren soils in the vicinity of a Pb/Zn Smelter
Structure and function of microbial communities during the early stages of revegetation of barren soils in the vicinity of a Pb/Zn Smelter
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DOI：
10.1016/j.geoderma.2006.04.011
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发表时间：
2006-12
期刊：
影响因子：
6.1
通讯作者：
Chong-bang Zhang;Li-Nan Huang;Tiangang Luan;Jing Jin;C. Lan
中科院分区：
文献类型：
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作者：
Chong-bang Zhang;Li-Nan Huang;Tiangang Luan;Jing Jin;C. Lan
为了确定微生物群落结构和功能是否可以作为非生物环境变化的指标，在中国南部亚热带条件下，对冶炼活动导致的工业荒地和以乡土植物泡桐为主的3个植被恢复点的微生物群落结构和功能(表示为磷脂脂肪酸)和功能(表示为酶活性)进行了研究。以自然植被覆盖下的邻近区域为对照。植被重建并未导致磷脂脂肪酸(PLFAs)和酶谱随时间的变化。而革兰氏阴性菌(16：1ω9c)、丛枝菌根真菌(16：1ω5c)、真菌(18：2ω6c)、放线菌(10Me16：0+10Me17：0+10Me18：0)、饱和脂肪酸(18：0)、单不饱和脂肪酸、藻类(20：5ω3c)、原生动物(20：2ω6c+20：3ω6c)等PLFA水平，以及包括蛋白酶、CM-纤维素酶和β-葡萄糖苷酶在内的酶活性在植被恢复后持续增加。与植被恢复后土壤全氮、pH和孔隙度的增加相对应。此外，环丙基脂肪酸/单烯酸前体(Cy17：0/16：1ω7c和Cy19：0/18：1ω7c)在恢复植被后的一段时间内显著下降，并与上述土壤理化参数呈负相关。主成分分析(PCA)表明，无论是PLFA数据还是酶活性数据，随着恢复时间的推移，三个恢复点都与对照点分离。典型相关分析(CCA)证实，在植被恢复过程中，微生物群落结构与其功能密切相关。结果表明，以白杨为主的植被覆盖显著改善了土壤微生物群落的结构和功能，对修复受重金属污染的工业荒地是有效的。在本研究确定的生物和非生物因素中，土壤物理化学参数(速效P和含水率除外)可能是决定土壤微生物群落结构和功能的更重要的因素，而不是恢复过程中的重金属含量。
Microbial community structure (expressed as phospholipid fatty acids) and function (expressed as enzyme activities) were studied in an industrial barren resulting from smelting activities, and three revegetated sites dominated by native plant Paulownia fortunei under the subtropical conditions of Southern China in order to determine whether microbial community structure and function can be used as indicators of changes in abiotic environment during the revegetation process. An adjacent area under natural vegetative cover was used as a control. The revegetation did not lead to consistent shifts of individual phospholipid fatty acids (PLFAs) and enzyme profiles with time. However, the levels of some signature PLFAs, such as Gram negative bacteria (16:1ω9c), arbuscular mycorrhizal (AM) fungi (16:1ω5c), fungi (18:2ω6c), actinomycetes (10Me16:0+10Me17:0+10Me18:0), saturated fatty acid (18:0), monounsaturated fatty acids (16:19c+16:1ω7c+16:1ω5c+17:1ω8c+18:1ω7c+18:1ω9c), algae (20:5ω3c), protozoa (20:2ω6c+20:3ω6c), PLFA ratios including fung/bact and monounsat/sat fatty acids, and enzyme activities including protease, CM-cellulase and β-glucosidase consistently increased with time after revegetation, corresponding to the increase in total N, pH and porosity in the revegetated soils. Moreover, cyclopropyl fatty acids/monoenoic precursors (cy17:0/16:1ω7c and cy19:0/18:1ω7c) significantly decreased during time after revegetation and was inversely correlated with the above soil physico-chemical parameters. Principal component analysis (PCA) indicated that the three revegetated sites were separated from the control sites with time after revegetation based on either the PLFA or enzyme activity data. Canonical correlation analysis (CCA) confirmed that the microbial community structure was closely linked to its function during the revegetation. Our data showed that the revegetative cover dominated by P. fortunei had considerably improved the structure and function of the soil microbial community, and was thus effective for remediation of industrial barrens contaminated with heavy metals. Of the biotic and abiotic factors determined in the present study, soil physico-chemical parameters (except for available P and WHC) may be more important factors in determining the structure and function of soil microbial community than heavy metal contents during the revegetation.