Identification of α 1-, α 2- and α 3-subunit isoforms of the GABAA-benzodiazepine receptor in the rat brain
Identification of α
1-, α
2- and α
3-subunit isoforms of the GABAA-benzodiazepine receptor in the rat brain
复制标题
大鼠脑中 GABAA-苯二氮卓受体 α 1-、α 2- 和 α 3-亚基亚型的鉴定
DOI：
10.1016/0304-3940(91)90470-e
复制
发表时间：
1991
影响因子：
2.5
通讯作者：
W. Sieghart
中科院分区：
文献类型：
--
作者：
A. Buchstaller;K. Fuchs;W. Sieghart
用N-聚糖酶和/或神经氨酸酶加O-聚糖酶将从5- 10日龄大鼠脑中纯化的GABA A受体完全N-和O-去糖基化。对完整或完全去糖基化的受体进行SDS-聚丙烯酰胺凝胶电泳和Western印迹分析。针对GABAA受体α1-、α2-或α3-亚基特异性合成氨基酸序列的多克隆抗体在完整受体中分别鉴定出约51 kDa、53 kDa或59 kDa的明显单一蛋白质。然而，在去糖基化受体中，通过针对α3亚基的抗体鉴定了三种不同的蛋白质，并且通过针对α2或α1亚基的抗体鉴定了至少两种不同的蛋白质。
GABAAreceptors purified from the brains of 5- to 10-day-old rats were completelyN- andO-deglycosylated usingN-glycanase and/or neuraminidase plusO-glycanase. Intact or completely deglycosylated receptors were subjected to SDS-polyacrylamide gel electrophoresis and Western blot analysis. Polyclonal antibodies directed against synthetic aminoacid sequences specific for the GABAAreceptorα1-,α2- orα3-subunits each identified an apparently single protein of about 51 kDa, 53 kDa or 59 kDa, respectively, in the intact receptors. In the deglycosylated receptors, however, three different proteins were identified by antibodies directed against theα3-subunit and at least two different proteins were identified by antibodies directed against theα2- orα1-subunit.
DOI：
--
发表时间：
1984-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
T. Plummer;J. Elder;S. Alexander;A. W. Phelan;A. Tarentino
通讯作者：
T. Plummer;J. Elder;S. Alexander;A. W. Phelan;A. Tarentino