Multi-site stimulation quiets network-wide spontaneous bursts and enhances functional plasticity in cultured cortical networks.
Multi-site stimulation quiets network-wide spontaneous bursts and enhances functional plasticity in cultured cortical networks.
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多部位刺激可以平息整个网络的自发爆发，并增强培养的皮质网络的功能可塑性。
DOI：
10.1109/iembs.2006.260571
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Potter,SteveM
中科院分区：
文献类型：
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作者：
Madhavan,Radhika;Chao,ZenasC;Wagenaar,DanielA;Bakkum,DouglasJ;Potter,SteveM
我们在多电极阵列 (MEA) 上培养高密度皮层培养物，这使我们能够刺激和记录数千个神经元。这些高密度培养物中的活动模式之一是培养皿范围内的同步爆裂。与体内不同的是，这些同步模式在培养物的一生中持续存在。这种异常的活动模式可能是由于皮层文化的感觉被剥夺和发育停滞所致。我们设计了通过多电极电刺激来控制这种自发活动的方法，并在这种突发安静刺激的背景下研究长期功能性神经可塑性。在这里，我们研究突发安静是否揭示了强直刺激引起的长期可塑性。由探针脉冲产生的时空活动模式（STAP）在安静和非安静的培养物中进行聚类和量化。与允许表达自发爆发的培养物相比，爆发安静的培养物表现出更多的破伤风诱导的功能变化。本研究开发的方法将有助于理解网络动态并了解其在大脑长期可塑性和信息处理中的作用。
We culture high-density cortical cultures on multi-electrode arrays (MEAs), which allow us to stimulate and record from thousands of neurons. One of the modes of activity in these high-density cultures is dish-wide synchronized bursting. Unlike in vivo, these synchronized patterns persist for the lifetime of the culture. Such aberrant patterns of activity might be due to the fact that cortical cultures are sensory-deprived and arrested in development. We have devised methods to control this spontaneous activity by multi-electrode electrical stimulation and to study long-term functional neural plasticity, on a background of such burst-quieting stimulation. Here, we investigate whether burst quieting reveals long-term plasticity induced by tetanic stimulation. Spatio-temporal activity patterns (STAPs) that result from probe pulses were clustered and quantified in quieted and non-quieted cultures. Burst-quieted cultures show more tetanus-induced functional change than cultures which are allowed to express spontaneous bursts. The methods developed for this study will help in the understanding of network dynamics and appreciation of their role in long-term plasticity and information processing in the brain.