3D printed magnesium-doped ?-TCP gyroid scaffold with osteogenesis, angiogenesis, immunomodulation properties and bone regeneration capability in vivo
3D printed magnesium-doped ?-TCP gyroid scaffold with osteogenesis, angiogenesis, immunomodulation properties and bone regeneration capability in vivo
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DOI：
10.1016/j.bioadv.2022.212759
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发表时间：
2022-05-25
影响因子：
--
通讯作者：
Xiao, Jun
中科院分区：
文献类型：
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作者：
Qi, Dahu;Su, Jin;Xiao, Jun
生物陶瓷已用于骨科手术多年。镁（Mg）是骨组织中的必需元素，在骨代谢中起着重要作用。掺镁生物陶瓷近年来引起了研究者的关注。然而，β-TCP中Mg的最佳掺杂量以及Mg掺杂的β-TCP（Mg-TCP）的免疫调节性质尚未确定。本研究采用数字光处理（DLP）技术制备了不同氧化镁含量（0、1、3、5 wt%）的β-TCP螺旋状支架材料，并对其理化性能和生物学性能进行了研究。Mg掺杂改善了β-TCP支架的物理化学性质。体外实验证实，Mg掺杂的β-TCP支架能促进骨髓间充质干细胞（BMSCs）的成骨分化和内皮祖细胞（EPCs）的血管分化，其中5 Mg-TCP在“单细胞型”方法中具有最佳性能。所有Mg-TCP均促进RAW264.7细胞向M2表型极化，尤其是3 Mg-TCP。然而，当使用“多细胞类型”方法时，3 Mg-TCP显示出最佳的成骨和血管生成潜力，该方法指的是在巨噬细胞条件培养基中培养BMSC或EPCs。最后，进行了体内实验，结果证实，3 Mg-TCP支架具有令人满意的骨缺损修复能力，在植入后6周和12周。这项研究表明，3 Mg-TCP支架提供了最佳的生物学性能，因此具有临床转化的潜力。
Bioceramics have been used in orthopedic surgery for several years. Magnesium (Mg) is an essential element in bone tissue and plays an important role in bone metabolism. Mg-doped bioceramics has attracted the attention of researchers recently. However, the optimal doping amount of Mg in beta-TCP and the immunomodulatory property of Mg-doped beta-TCP (Mg-TCP) have not been determined yet. In this study, beta-TCP scaffolds doped with different contents of magnesium oxide (0 wt%, 1 wt%, 3 wt%, and 5 wt%) with gyroid structure were printed by digital light processing (DLP) method, and the physicochemical and biological functions were then investigated. Mgdoping improved the physicochemical properties of the beta-TCP scaffolds. In vitro experiments confirmed that the doping of Mg in beta-TCP scaffolds promoted the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and angiogenic differentiation of endothelial progenitor cells (EPCs), where the 5Mg-TCP has the optimal properties when using the "one cell type" method. It was also found that all Mg-TCP facilitated the polarization of RAW264.7 cells to the M2 phenotype, especially the 3Mg-TCP. However, 3Mg-TCP displayed the optimal osteogenic and angiogenic potential when using a "multiple cell type" method, which referred to culturing the BMSCs or EPCs in the macrophage-conditioned medium. Finally, the in vivo experiments were conducted and the results confirmed that the 3Mg-TCP scaffolds possessed the satisfying bone defect repair capability both after 6 and 12 weeks of implantation. This study suggests that 3Mg-TCP scaffolds provide the optimal biological performance and thus have the potential for clinical translation.