Karyotype stability of the DT40 chicken B cell line: Macrochromosome variation and cytogenetic mosaicism
Karyotype stability of the DT40 chicken B cell line: Macrochromosome variation and cytogenetic mosaicism
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DOI：
10.1023/b:chro.0000021947.17128.96
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发表时间：
2004-01-01
影响因子：
2.6
通讯作者：
Delany, ME
中科院分区：
文献类型：
--
作者：
Chang, H;Delany, ME
DT 40转化的鸡B细胞系是一种重要且广泛使用的脊椎动物细胞系。对于大多数研究应用和将结果外推到正常细胞来说，了解“野生型”DT 40核型是一个重要的考虑因素。在这里，我们提出的数据表明：（1）模态核型差异存在于不同来源的DT 40培养物之间，（2）DT 40培养物是细胞遗传学嵌合体，（3）嵌合体特征可以随着时间的推移而改变，培养基条件影响出现的非模态核型的程度和类型。除了先前报道的2号染色体三体，4号染色体的单体性和4p-的变体进行了描述。DT 40系统的宏染色体变异和镶嵌性质应提醒使用这一重要细胞系的研究人员考虑这种变异是否会影响实验结果和解释。结果进行了讨论，就目前的知识与鸡癌症的细胞遗传学异常。
The DT40 transformed chicken B-cell line is an important and widely used vertebrate cell line. Knowledge of the `wild-type' DT40 karyotype is a significant consideration for most research applications and for extrapolation of results to normal cells. Here we present data indicating that (1) modal karyotype differences exist between DT40 cultures from different sources, (2) DT40 cultures are cytogenetically mosaic, and (3) the mosaic features can change over time with media conditions influencing the degree and type of non-modal karyotypes that emerge. In addition to the previously reported trisomy for chromosome 2, a monosomy for chromosome 4 and a variant 4p- are described. The macrochromosomal variation and mosaic nature of the DT40 system should alert researchers using this important cell line to consider whether such variation will impact experimental results and interpretation. The results are discussed in regard to current knowledge of cytogenetic anomalies associated with chicken cancers.