Gene expression profile during ovarian folliculogenesis
Gene expression profile during ovarian folliculogenesis
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DOI：
10.1016/j.jri.2009.09.002
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发表时间：
2009-12-01
影响因子：
3.4
通讯作者：
Koyama, K.
中科院分区：
文献类型：
--
作者：
Hasegawa, A.;Kumamoto, K.;Koyama, K.
辅助生殖技术取得了重大进展，为许多不孕夫妇提供了成功的治疗。然而，对于严重不孕症，如卵巢早衰和癌症辅助治疗引起的卵巢功能损害，需要更先进的技术。卵巢组织冷冻保存，然后在体外生长的孤立卵泡是一个可行的主张，为这样的患者。卵泡生长需要卵泡细胞（包括卵母细胞、颗粒细胞和卵泡膜细胞）之间的密切协调沟通。促性腺激素非依赖性卵泡和促性腺激素依赖性卵泡的生长阶段可能受到一些关键因素的调节。为了促进早期生长卵泡的培养系统的开发，对在7、10、13、16和19日龄时回收的小鼠卵巢进行DNA微阵列分析，以鉴定早期卵泡生长所需的因素。这些研究表明，在7日龄小鼠中，透明质酸糖蛋白、骨形态发生蛋白-15（BMP-15）和生长分化因子（GDF-9）的强度很强，在19日龄小鼠中逐渐下降。KIT、KIT配体、抗苗勒管激素（AMH）和血小板衍生生长因子（PDGF），被称为颗粒细胞分泌因子，也显示出相对高的表达。这些研究将有助于我们了解卵泡发生的调控因素，从而为卵泡体外培养体系的建立奠定基础。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
Assisted reproductive technologies have progressed significantly and have provided successful treatment for many infertile couples. However, more advanced technologies are required for severe infertility such as premature ovarian failure and ovarian impairment due to adjuvant therapy for cancer. Ovarian tissue cryopreservation followed by in vitro growth of isolated follicles is a feasible proposition for such patients. Close coordination of communication among follicle cells including oocytes, granulosa and theca cells is required for follicle growth. Crucial factors may regulate the gonadotropin-independent and -dependent follicle growth stages. To facilitate development of a culture system for early growing follicles, DNA microarray analysis of mouse ovaries recovered at 7, 10, 13, 16 and 19 days of age was performed to identify factors required for the growth of early-stage follicles. These studies showed strong intensity of zona pellucida glycoproteins, bone morphogenic protein-15 (BMP-15) and growth differentiation factor (GDF-9) in 7 days old mice, which gradually declined in 19 days old mice. KIT, KIT ligand, anti-mullerian hormone (AMH) and platelet-derived growth factor (PDGF), known as granulosa cell secreted factors, also showed relatively high expression. These studies will facilitate our understanding of the regulatory factors involved in folliculogenesis and thereby enable establishment of in vitro culture system for ovarian follicles. (C) 2009 Elsevier Ireland Ltd. All rights reserved.