Bipolar electrochemiluminescence on thread: A new class of electroanalytical sensors
Bipolar electrochemiluminescence on thread: A new class of electroanalytical sensors
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线上双极电化学发光：新型电分析传感器
DOI：
10.1016/j.bios.2017.03.007
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发表时间：
2017-08-15
影响因子：
12.6
通讯作者：
Zhang, Chunsun
中科院分区：
文献类型：
--
作者：
Liu, Rui;Liu, Cuiling;Zhang, Chunsun
本文介绍了一种新的和简单的概念，制造低成本，易于使用的毛细管微通道（CMC）辅助线程为基础的微流控分析装置（CMCA-mu TADs）的双极电化学发光（BP-ECL）的应用。将具有碳丝网印刷电极和裸线区域（BTZ）的图案的线嵌入CMC中。这种CMCA-mu TAD可以产生强而稳定的BP-ECL信号，并且具有极低的成本（每个器件0.01美元）。有趣的是，CMCA-mu TAD是超柔性的，并且可以在BTZ处以135 °弯曲角度或在双极电极（BPE）的中间以150 °弯曲角度弯曲，而不会损失分析性能。此外，我们还用Ru（bpy）（3）（2+）/TPA和鲁米诺/H2 O2两种常用的电化学发光体系来验证BP-ECL CMCA-mu TADs的定量能力。结果表明，所提出的装置已成功地实现了TPA和H2 O2的检测，检测限分别为0.00432 mM和0.00603 mM。在鲁米诺/H2 O2电化学发光体系的基础上，将CMCA-mu TADs进一步应用于葡萄糖的测定，其检测限为0.0205 mM，并将其应用于牛奶中H2 O2、人尿液和血清中葡萄糖的测定，结果表明CMCA-mu TADs具有良好的应用性和有效性。结果表明，所提出的设备有可能成为一个重要的新工具，广泛的应用。
This paper introduces a new and simple concept for fabricating low-cost, easy-to-use capillary microchannel (CMC) assisted thread-based microfluidic analytical devices (CMCA-mu TADs) for bipolar electrochemiluminescence (BP-ECL) application. The thread with patterns of carbon screen-printed electrodes and bare thread zones (BTZs) is embedded into a CMC. Such CMCA-mu TADs can produce a strong and stable BP-ECL signal, and have an extremely low cost ($0.01 per device). Interestingly, the CMCA-mu TADs are ultraflexible, and can be bent with a 135 bending angle at the BTZ or with a 150 bending angle at the middle of bipolar electrode (BPE), with no loss of analytical performance. Additionally, the two commonly-used ECL systems of Ru(bpy)(3)(2+)/TPA and luminol/H2O2 are applied to demonstrate the quantitative ability of the BP-ECL CMCA-mu TADs. It has been shown that the proposed devices have successfully fulfilled the detection of TPA and H2O2, with detection limits of 0.00432 mM and 0.00603 mM, respectively. Based on the luminol/H2O2 ECL system, the CMCA- mu TADs are further applied for the glucose measurement, with the detection limit of 0.0205 mM. Finally, the applicability and validity of the CMCA-mu TADs are demonstrated for the measurements of H2O2 in milk, and glucose in human urine and serum. The results indicate that the proposed devices have the potential to become an important new tool for a wide range of applications.