BMPs induce direct bone formation in ectopic sites independent of the endochondral ossification in vivo
BMPs induce direct bone formation in ectopic sites independent of the endochondral ossification in vivo
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BMP 在异位部位诱导直接骨形成，与体内软骨内骨化无关
DOI：
10.1002/ar.1092360211
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Y. Kuboki
中科院分区：
文献类型：
--
作者：
Y. Sasano;Eriko Ohtani;K. Narita;M. Kagayama;M. Murata;Takashi Saito;K. Shigenobu;H. Takita;M. Mizuno;Y. Kuboki
体内骨形成通过两个主要过程发生，其中一个依赖于预先存在的软骨，另一个不依赖于预先存在的软骨。骨形态发生蛋白（BMP）已被认为可诱导非成骨间充质细胞群形成软骨，这导致通过软骨内序列的成骨。在本研究中，我们使用牛纤维胶原膜（FCM）作为BMP的载体，研究了BMP是否可以独立于预先存在的软骨直接形成骨。在4 M盐酸胍中提取牛跖骨，并通过羟基磷灰石色谱法和肝素-琼脂糖CL 6 B色谱法部分纯化BMP。载体负载BMP，然后植入Wistar大鼠皮下。植入后每周将植入物与周围组织固定在一起，并进行von Kossa染色、免疫组织化学和电子显微镜检查。骨和软骨的表型进行了鉴定，并使用抗I型和II型胶原抗体的组织化学。软骨和骨独立诱导2周。骨直接在FCM的胶原基质上形成，而没有预先存在的软骨。钙化发生在载体以及软骨和骨基质中。目前的研究表明，骨形成蛋白诱导成骨在体内独立的endochondrial序列。© 1993 Wiley利斯公司
Bone formation in vivo occurs via two major processes, one of which depends on pre‐existing cartilage, and the other does not. Bone morphogenetic proteins (BMPs) have been suggested to induce cartilage formation from non‐skeletogenic mesenchymal cell population, which results in osteogenesis through the endochondral sequence. In the present study we examined if BMPs could cause direct bone formation independent of pre‐existing cartilage using bovine fibrous collagen membrane (FCM) as a carrier for BMPs. Bovine metatarsal bone was extracted in 4 M guanidine HCl and BMPs were partially purified through the hydroxyapatite chromatography and the Heparin‐Sepharose CL6B chromatography. The carrier was loaded with BMPs and then implanted in Wistar rats subcutaneously. The implants were fixed together with surrounding tissue every week after implantation and processed for von Kossa stain, immunohistochemistry, and electron microscopy. The phenotypes of bone and cartilage were identified histologically and immunohistochemically using antibodies against type I and type II collagen. Cartilage and bone were independently induced by 2 weeks. The bone formed directly on the collagen substrate of FCM without pre‐existing cartilage. Calcification occurred in the carrier as well as the cartilage and bone matrix. The present study suggests that the BMPs induce osteogenesis in vivo independent of the endochondral sequence. © 1993 Wiley‐Liss, Inc.
破骨细胞在大鼠体内吸收脱矿质和含矿物质骨植入物的特征。
DOI：
--
发表时间：
1989
期刊：
Scanning microscopy
影响因子：
--
作者：
Bagi,CM;Miller,SC
通讯作者：
Miller,SC