The expression of interleukin-32 is activated by human cytomegalovirus infection and down regulated by hcmv-miR-UL112-1.
The expression of interleukin-32 is activated by human cytomegalovirus infection and down regulated by hcmv-miR-UL112-1.
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IL-32的表达被人巨细胞病毒感染激活并被hcmv-miR-UL112-1下调
DOI：
10.1186/1743-422x-10-51
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发表时间：
2013-02-12
期刊：
影响因子：
4.8
通讯作者：
Ruan Q
中科院分区：
文献类型：
--
作者：
Huang Y;Qi Y;Ma Y;He R;Ji Y;Sun Z;Ruan Q
白介素-32（IL-32）是先天和适应性免疫调查的重要因素，它激活了p38mapk，NF-kappa b和ap-1信号通路。
进行了酶联的免疫吸附测定（ELISA），以检测血清样品中的IL-32水平。在96小时的时间课程中使用Taqman®MiRNA分析进行调节。 HCMV-MIR-UL112-1对IL-32表达的影响分别通过荧光素酶测定和蛋白质印迹证明了。
HCMV-IGM阳性患者的血清32水平（表明活跃的HCMV感染）明显高于HCMV-IgM阴性对照中的HCMV感染。表达HCMV-MIR-UL112-1在24 hpi下逐渐增加，随着HCMV感染过程的延长，还表明HCMV-MIR-UL112-1靶向IL-32 32 3'-UTR中的IL-32蛋白质水平的IL-32中的IL-32中的序列。
IL-32的表达是由活性HCMV感染诱导的，并且可以通过经济表达的HCMV-MIR-UL112-1在功能上下调。
BackgroundInterleukin-32 (IL-32) is an important factor in innate and adaptive immune responses, which activates the p38MAPK, NF-kappa B and AP-1 signaling pathways. Recent reports have highlighted that IL-32 is regulated during viral infection in humans.MethodsEnzyme-linked immunosorbent assays (ELISA) were carried out to detect IL-32 levels in serum samples. Detailed kinetics of the transcription of IL-32 mRNA and expression of IL-32 protein during human cytomegalovirus (HCMV) infection were determined by semi-quantitative RT-PCR and western blot, respectively. The expression levels of hcmv-miR-UL112-1 were detected using TaqMan® miRNA assays during a time course of 96 hours. The effects of hcmv-miR-UL112-1 on IL-32 expression were demonstrated by luciferase assay and western blot, respectively.ResultsSerum levels of IL-32 in HCMV-IgM positive patients (indicating an active HCMV infection) were significantly higher than those in HCMV-IgM negative controls. HCMV infection activated cellular IL-32 transcription mainly in the immediately early (IE) phase and elevated IL-32 protein levels between 6 and 72 hours post infection (hpi) in the human embryonic lung fibroblast cell line, MRC-5. The expression of hcmv-miR-UL112-1 was detected at 24 hpi and increased gradually as the HCMV-infection process was prolonged. In addition, it was demonstrated that hcmv-miR-UL112-1 targets a sequence in the IL-32 3′-UTR. The protein level of IL-32 in HEK293 cells could be functionally down-regulated by transfected hcmv-miR-UL112-1.ConclusionsIL-32 expression was induced by active HCMV infection and could be functionally down-regulated by ectopically expressed hcmv-miR-UL112-1. Our data may indicate a new strategy of immune evasion by HCMV through post-transcriptional regulation.