Optimising ultrasound-mediated gene transfer (sonoporation) in vitro and prolonged expression of a transgene in vivo: Potential applications for gene therapy of cancer
Optimising ultrasound-mediated gene transfer (sonoporation) in vitro and prolonged expression of a transgene in vivo: Potential applications for gene therapy of cancer
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DOI：
10.1016/j.canlet.2008.07.030
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发表时间：
2009-01-08
期刊：
影响因子：
9.7
通讯作者：
McHale, Anthony P.
中科院分区：
文献类型：
--
作者：
Li, Ying Suet;Davidson, Emma;McHale, Anthony P.
使用基因药物的治疗方法有望替代或取代传统的癌症治疗。由于其非侵入性的性质，超声波作为膜透化刺激具有与病毒基因递送和现有的非病毒替代品高度竞争的潜力。优化超声介导的微泡辅助（MB 101）体外基因转染。我们证明了在1 MHz、占空比为25%、强度范围为1至4 W cm（-2）的情况下使用超声的效率高达18%。使用超声波介导的转染与附加型质粒为基础的基因表达系统，我们证明了在小鼠后腿肌肉和肿瘤在体内荧光素酶的功能基因表达延长。皇冠版权所有（C）2008由爱思唯尔有限公司出版。保留所有权利。
Therapeutic approaches using gene-based medicines promise alternatives or adjuncts to conventional cancer treatment. Because of its non-invasive nature, ultrasound, as a membrane-permeabilising stimulus has the potential to be highly competitive with viral gene delivery and existing non-viral alternatives. In optimising ultrasound-mediated, microbubble-assisted (MB101) gene tranfection in vitro. we demonstrate efficiencies of up to 18% using ultrasound at 1 MHz at a duty cycle of 25% at intensities ranging from 1 to 4 W cm(-2). Using ultrasound-mediated transfection together with an episomal plasmid-based gene expression system, we demonstrate prolonged functional gene expression of luciferase in mouse hind leg muscle and in tumours in vivo. Crown Copyright (C) 2008 Published by Elsevier Ltd. All rights reserved.