mini spindles:: A gene encoding a conserved microtubule-associated protein required for the integrity of the mitotic spindle in Drosophila
mini spindles:: A gene encoding a conserved microtubule-associated protein required for the integrity of the mitotic spindle in Drosophila
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DOI：
10.1083/jcb.146.5.1005
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发表时间：
1999-09-06
影响因子：
7.8
通讯作者：
Ohkura, H
中科院分区：
文献类型：
--
作者：
Cullen, CF;Deák, P;Ohkura, H
我们描述了一种新的果蝇基因，迷你纺锤体(MSPs)，在有丝分裂突变的细胞学筛查中被发现。MSPs的突变破坏了有丝分裂纺锤体的结构完整性，导致在二倍体细胞中形成一个或多个额外的小纺锤体。来自中心体的微管成核、染色体的中期排列或纺锤体极的聚焦似乎受到的影响要小得多。MSPs基因编码一个227 kD的蛋白，与脊椎动物微管相关蛋白(MAPs)、人TOGp和非洲爪哇XMAP215高度相似，与分裂酵母和芽生酵母的Dis1和STU2蛋白相似性有限。与它们的序列相似性一致，MSPs蛋白在体外也与微管结合。在胚胎分裂周期中，MSPs蛋白在所有有丝分裂阶段定位于中心体区，中期和后期分布于纺锤体。细胞化胚胎间期未见中心体染色，提示MSPs蛋白与微管或中心体的相互作用可能在细胞周期中受到调控。
We describe a new Drosophila gene, mini spindles (msps) identified in a cytological screen for mitotic mutant. Mutation in msps disrupts the structural integrity of the mitotic spindle, resulting in the formation of one or more small additional spindles in diploid cells. Nucleation of microtubules from centrosomes, metaphase alignment of chromosomes, or the focusing of spindle poles appears much less affected. The msps gene encodes a 227-kD protein with high similarity to the vertebrate microtubule-associated proteins (MAPs), human TOGp and Xenopus XMAP215, and with limited similarity to the Dis1 and STU2 proteins from fission yeast and budding yeast. Consistent with their sequence similarity, Msps protein also associates with microtubules in vitro. In the embryonic division cycles, Msps protein localizes to centrosomal regions at ail mitotic stages, and spreads over the spindles during metaphase and anaphase. The absence of centrosomal staining in interphase of the cellularized embryos suggests that the interactions between Msps protein and microtubules or centrosomes may be regulated during the cell cycle.