Comparison of the genetic structure of populations of wild emmer wheat, Triticum turgidum ssp dicoccoides, from Israel and Turkey revealed by AFLP analysis
Comparison of the genetic structure of populations of wild emmer wheat, Triticum turgidum ssp dicoccoides, from Israel and Turkey revealed by AFLP analysis
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DOI：
10.1007/s10722-006-9171-8
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发表时间：
2007-11-01
影响因子：
2
通讯作者：
Feldman, M.
中科院分区：
文献类型：
--
作者：
Ozbek, O.;Millet, E.;Feldman, M.
本研究的目的是评估在以色列和土耳其的野生二粒小麦，turgidum ssp的几个群体的遗传变异。大多数驯化小麦的祖先。2002年，从以色列东北部阿米亚德的6个不同栖息地生长的60株植物中收集了单穗（每个栖息地8-12株植物），并于1998年从土耳其东南部迪亚巴基尔的7个不同栖息地生长的56株植物中收集了单穗（每个栖息地8株植物）。将种子种植在苗圃中，从每株植物中提取DNA，并通过基于荧光的扩增片段长度多态性（AFLP）方法进行分析。7个引物组合产生788个可辨别的位点，其中48.6%在以色列和40.5%在土耳其的多态性。Ammiad的遗传多样性估计值P（多态位点频率）和He（基因多样性）高于迪亚巴基尔（Ammiad的平均值P = 0.34和He = 0.13，而迪亚巴基尔的平均值P = 0.20和He = 0.08）。Ammiad群体比迪亚巴基尔群体含有更多的独特等位基因。相对遗传多样性估计（θ）值分别为0.188在Ammiad和0.407在迪亚巴基尔，这表明更好的分化在土耳其的人口。以色列和土耳其人口之间的遗传距离大于每个国家的人口之间的遗传距离。这些数据表明，以色列和土耳其的人口是相当分歧的，以色列人口比土耳其的多态性，有一个更大的群体内的遗传变异比ESTA人口之一。本文还讨论了这些结果对近东地区野生二粒小麦分化模式的意义。
The aim of the present study was to assess the genetic variation in several Israeli and Turkish populations of wild emmer wheat, Triticum turgidum ssp. dicoccoides, the progenitor of most domesticated wheat. Single spikes were collected in 2002 from 60 plants that grew in six different habitats in Ammiad, northeastern Israel (8-12 plants from each habitat), and in 1998 from 56 plants that grew in seven different habitats in Diyarbakir, southeastern Turkey (8 plants from each habitat). Seeds were planted in a nursery and DNA was extracted from every plant and analyzed by the fluorescent-based amplified fragment length polymorphism (AFLP) method. Seven primer combinations produced 788 discernible loci of which 48.6% were polymorphic in Israel and 40.5% in Turkey. The genetic diversity estimates P (frequency of polymorphic loci) and He (gene diversity) were higher in Ammiad than in Diyarbakir (means of P = 0.34 and He = 0.13 in Ammiad vs. P = 0.20 and He = 0.08 in Diyarbakir). Ammiad populations contained more unique alleles than Diyarbakir populations. The relative genetic diversity estimates (theta) values were 0.188 in Ammiad and 0.407 in Diyarbakir, suggesting better differentiation of the populations in Turkey. Genetic distance was larger between Israeli and Turkish populations than between populations of each country. The data indicate that the Israeli and Turkish populations are considerably diverged and that the Israeli populations are more polymorphic than the Turkish ones, having a larger within-populations genetic variation than among-populations one. The significance of the results in relation to the differentiation pattern of wild emmer in the Near East is discussed.