Computational 3D microscopy with optical coherence refraction tomography.
Computational 3D microscopy with optical coherence refraction tomography.
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DOI：
10.1364/optica.454860
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发表时间：
2022-02
期刊：
影响因子：
10.4
通讯作者：
Kevin C Zhou;R. McNabb;Ruobing Qian;S. Degan;Al-Hafeez Z. Dhalla;Sina Farsiu;J. Izatt
中科院分区：
文献类型：
--
作者：
Kevin C Zhou;R. McNabb;Ruobing Qian;S. Degan;Al-Hafeez Z. Dhalla;Sina Farsiu;J. Izatt
光学相干断层扫描（OCT）作为体内临床诊断3D成像模式已经取得了广泛的成功，影响了包括眼科学、心脏病学和胃肠病学在内的领域。尽管OCT具有许多优点，例如高灵敏度、速度和深度穿透，但OCT存在几个缺点，最终限制了其作为3D显微镜工具的实用性，例如其普遍存在的相干散斑噪声和维持毫米级成像深度所需的不良横向分辨率。在这里，我们提出了3D光学相干折射断层扫描（OCRT），OCT的计算扩展，通过结合多个OCT体积，跨两个旋转轴采集，形成一个分辨率增强，斑点减少，折射校正的3D重建合成非相干对比机制。我们的无标记计算3D显微镜采用了一种新颖的光学设计，结合了抛物面镜，能够捕获5D全光数据集，包括毫米级3D视场，最高可达± 75 °，无需移动样品。我们证明，3D OCRT揭示了果蝇，斑马鱼和小鼠样本中常规OCT未观察到的3D特征。
Optical coherence tomography (OCT) has seen widespread success as an in vivo clinical diagnostic 3D imaging modality, impacting areas including ophthalmology, cardiology, and gastroenterology. Despite its many advantages, such as high sensitivity, speed, and depth penetration, OCT suffers from several shortcomings that ultimately limit its utility as a 3D microscopy tool, such as its pervasive coherent speckle noise and poor lateral resolution required to maintain millimeter-scale imaging depths. Here, we present 3D optical coherence refraction tomography (OCRT), a computational extension of OCT which synthesizes an incoherent contrast mechanism by combining multiple OCT volumes, acquired across two rotation axes, to form a resolution-enhanced, speckle-reduced, refraction-corrected 3D reconstruction. Our label-free computational 3D microscope features a novel optical design incorporating a parabolic mirror to enable the capture of 5D plenoptic datasets, consisting of millimetric 3D fields of view over up to ±75° without moving the sample. We demonstrate that 3D OCRT reveals 3D features unobserved by conventional OCT in fruit fly, zebrafish, and mouse samples.