Effect of amino acid substitution in the hydrophobic face of amphiphilic peptides on membrane curvature and perturbation: N‐terminal helix derived from adenovirus internal protein VI as a model
Effect of amino acid substitution in the hydrophobic face of amphiphilic peptides on membrane curvature and perturbation: N‐terminal helix derived from adenovirus internal protein VI as a model
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两亲肽疏水面氨基酸取代对膜曲率和扰动的影响：以腺病毒内部蛋白 VI 衍生的 N 端螺旋为模型
DOI：
10.1002/bip.22797
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发表时间：
2016
期刊：
影响因子：
2.4
通讯作者：
S. Futaki
中科院分区：
文献类型：
--
作者：
T. Murayama;S. Pujals;Hisaaki Hirose;I. Nakase;S. Futaki
腺病毒内部蛋白VI（AdVpVI）的N末端两亲性螺旋片段在病毒感染中起关键作用。在这里，我们报告的肽段对应于AdVpVI（位置33-55）可以诱导积极的膜曲率与膜扰动。增强扰动能力的肽被观察到的膜含有带负电荷的磷脂。基于脂质体渗漏试验，取代亮氨酸在位置40的其他脂肪族（异亮氨酸）和芳香族（苯丙氨酸和色氨酸）残基产生了类似程度的膜扰动的肽，这是相当大的减少取代谷氨酰胺。使用野生型AdVpVI（33-55）（WT）和苯丙氨酸取代的肽（L40 F）的进一步研究表明，两种肽都具有正膜曲率诱导能力。这些肽对50 nm大单层囊泡（LUV）的结合亲和力高于200 nm LUV。然而，与200 nm LUV相比，50 nm LUV没有观察到这些肽的干扰增强，这表明原始膜曲率和由于肽插入引起的额外应变都会影响这些肽的膜干扰能力。在L40 F的情况下，该肽对50 nm LUV的膜扰动能力比对200 nm LUV的膜扰动能力更低，这可以归因于L40 F在膜上的结合可能更浅。© 2016 Wiley Periodicals，Inc. Biopolymers（Pept Sci）106：430-439，2016.
The N‐terminal amphipathic helical segment of adenovirus internal protein VI (AdVpVI) plays a critical role in viral infection. Here, we report that the peptide segment corresponding to AdVpVI (positions 33–55) can induce positive membrane curvature together with membrane perturbation. The enhanced perturbation ability of the peptide was observed for membranes containing negatively charged phospholipids. Based on the liposome leakage assay, substitution of leucine at position 40 to other aliphatic (isoleucine) and aromatic (phenylalanine and tryptophan) residues yielded a similar degree of membrane perturbation by the peptides, which was considerably diminished by the substitution to glutamine. Further studies using the wild‐type AdVpVI (33–55) (WT) and phenylalanine‐substituted peptides (L40F) demonstrated that both peptides have positive membrane‐curvature‐inducing ability. These peptides showed higher binding affinity to 50‐nm large unilamellar vesicles (LUVs) than to 200‐nm LUVs. However, no enhanced perturbation by these peptides was observed for 50‐nm LUVs compared to 200‐nm LUVs, suggesting that both the original membrane curvature and the additional strain due to peptide insertion affect the membrane perturbation ability of these peptides. In the case of L40F, this peptide rather had a lower membrane perturbation ability for 50‐nm LUVs than for 200‐nm LUVs, which can be attributed to possible shallower binding of L40F on membranes. © 2016 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 106: 430–439, 2016.
影响因子：
3.4
作者：
Thennarasu, Sathiah;Tan, Anmin;Ramamoorthy, Ayyalusamy
通讯作者：
Ramamoorthy, Ayyalusamy
影响因子：
3.4
作者：
Reshetnyak, Yana K.;Segala, Michael;Engelman, Donald M.
通讯作者：
Engelman, Donald M.
影响因子：
3.4
作者：
Hallock, KJ;Lee, DK;Ramamoorthy, A
通讯作者：
Ramamoorthy, A