Rapid Evaporative Ionisation Mass Spectrometry (REIMS) Provides Accurate Direct from Culture Species Identification within the Genus Candida.
Rapid Evaporative Ionisation Mass Spectrometry (REIMS) Provides Accurate Direct from Culture Species Identification within the Genus Candida.
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快速蒸发电离质谱法（REIMS）从念珠菌属内的培养物种鉴定提供了准确的直接直接。
DOI：
10.1038/srep36788
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发表时间：
2016-11-14
影响因子：
4.6
通讯作者：
Takáts Z
中科院分区：
文献类型：
--
作者：
Cameron SJ;Bolt F;Perdones-Montero A;Rickards T;Hardiman K;Abdolrasouli A;Burke A;Bodai Z;Karancsi T;Simon D;Schaffer R;Rebec M;Balog J;Takáts Z
念珠菌属的成员，如白色念珠菌和假丝酵母菌，是重要的人类病原体。这一属的其他成员，以前被认为具有最小的疾病风险，越来越多地被认为是重要的人类病原体，特别是因为对广泛使用的抗真菌药物的敏感性存在差异。因此，快速准确地鉴定临床分离念珠菌是确保及时有效治疗的基础。快速蒸发电离质谱法（REIMS）先前已被证明为快速准确鉴定细菌和真菌分离物提供了高通量平台。与市售的基质辅助激光解吸电离飞行时间质谱（MALDI-ToF）相比，基于REIMS的方法不需要准备步骤，也不需要耗时的细胞提取。在这里，我们报告了基于reims的分析能够快速准确地识别153个临床念珠菌分离株到物种水平。手持式双极REIMS和高通量REIMS平台均显示出较高的物种分类准确率，分别有96%和100%的菌株被正确分类到物种水平。此外，在600 ~ 1000 m/z质量范围内，鉴定出显著差异（FDR校正P值< 0.05）的脂质，这些脂质可以作为复杂微生物群落的物种特异性生物标志物。
Members of the genus Candida, such as C. albicans and C. parapsilosis, are important human pathogens. Other members of this genus, previously believed to carry minimal disease risk, are increasingly recognised as important human pathogens, particularly because of variations in susceptibilities to widely used anti-fungal agents. Thus, rapid and accurate identification of clinical Candida isolates is fundamental in ensuring timely and effective treatments are delivered. Rapid Evaporative Ionisation Mass Spectrometry (REIMS) has previously been shown to provide a high-throughput platform for the rapid and accurate identification of bacterial and fungal isolates. In comparison to commercially available matrix assisted laser desorption ionisation time of flight mass spectrometry (MALDI-ToF), REIMS based methods require no preparative steps nor time-consuming cell extractions. Here, we report on the ability of REIMS-based analysis to rapidly and accurately identify 153 clinical Candida isolates to species level. Both handheld bipolar REIMS and high-throughput REIMS platforms showed high levels of species classification accuracy, with 96% and 100% of isolates classified correctly to species level respectively. In addition, significantly different (FDR corrected P value < 0.05) lipids within the 600 to 1000 m/z mass range were identified, which could act as species-specific biomarkers in complex microbial communities.
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影响因子：
14.2
作者：
Bader, O.;Weig, M.;Kuhns, M.
通讯作者：
Kuhns, M.
影响因子：
2.9
作者：
Fraser, Mark;Brown, Zoe;Johnson, Elizabeth M.
通讯作者：
Johnson, Elizabeth M.
影响因子：
11.8
作者：
JARVIS, WR
通讯作者：
JARVIS, WR
影响因子：
17.1
作者：
Balog, Julia;Sasi-Szabo, Laszlo;Takats, Zoltan
通讯作者：
Takats, Zoltan
影响因子：
6.5
作者：
Fahy, Eoin;Subramaniam, Shankar;Dennis, Edward A.
通讯作者：
Dennis, Edward A.