Computational fluid modeling and performance analysis of a bidirectional rotating perfusion culture system
Computational fluid modeling and performance analysis of a bidirectional rotating perfusion culture system
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双向旋转灌注培养系统的计算流体建模和性能分析
DOI：
10.1002/btpr.1736
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发表时间：
2013-07-01
影响因子：
2.9
通讯作者：
Leo, Hwa Liang
中科院分区：
文献类型：
--
作者：
Kang, Chang-Wei;Wang, Yan;Leo, Hwa Liang
无数的生物反应器配置已经被研究作为体外医疗支持系统暂时替代重要器官的功能。近年来的研究表明，旋转生物反应器具有易于扩展细胞培养量的优点，具有作为生物人工肝辅助装置（blad）的潜力。然而，旋转室中的流体运动将使悬浮细胞暴露于不需要的流动结构中，这些流动结构具有异常高的剪切条件，可能导致细胞稳定性差，进而降低生物反应器系统的效率。在这项研究中，我们比较了我们改进的旋转生物反应器设计与现有旋转生物反应器设计的水动力性能。将计算流体动力学分析与实验结果相结合，进行了改进生物反应器设计的优化过程。模拟结果表明，与传统设计相比，改进后的生物反应器具有更低的流体诱导剪切应力和更均匀的旋转腔内流动条件。实验结果表明，与传统设计相比，改性生物反应器中的细胞也表现出更好的细胞载体附着，更高的代谢活性和细胞活力。总之，该研究能够为旋转生物反应器内的流动物理提供重要的见解，并有助于提高现有旋转生物反应器在BLAD应用中的流体动力学性能。(c) 2013年美国化学工程师学会生物技术。掠夺。中文信息学报，29:1002-1012,2013
A myriad of bioreactor configurations have been investigated as extracorporeal medical support systems for temporary replacement of vital organ functions. In recent years, studies have demonstrated that the rotating bioreactors have the potential to be utilized as bioartificial liver assist devices (BLADs) owing to their advantage of ease of scalability of cell-culture volume. However, the fluid movement in the rotating chamber will expose the suspended cells to unwanted flow structures with abnormally high shear conditions that may result in poor cell stability and in turn lower the efficacy of the bioreactor system. In this study, we compared the hydrodynamic performance of our modified rotating bioreactor design with that of an existing rotating bioreactor design. Computational fluid dynamic analysis coupled with experimental results were employed in the optimization process for the development of the modified bioreactor design. Our simulation results showed that the modified bioreactor had lower fluid induced shear stresses and more uniform flow conditions within its rotating chamber than the conventional design. Experimental results revealed that the cells within the modified bioreactor also exhibited better cell-carrier attachment, higher metabolic activity, and cell viability compared to those in the conventional design. In conclusion, this study was able to provide important insights into the flow physics within the rotating bioreactors, and help enhanced the hydrodynamic performance of an existing rotating bioreactor for BLAD applications. (c) 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:1002-1012, 2013