Ectopic expression of wheat TaCIPK14, encoding a calcineurin B-like protein-interacting protein kinase, confers salinity and cold tolerance in tobacco
Ectopic expression of wheat TaCIPK14, encoding a calcineurin B-like protein-interacting protein kinase, confers salinity and cold tolerance in tobacco
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DOI：
10.1111/ppl.12046
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发表时间：
2013-11-01
影响因子：
6.4
通讯作者：
He, Guangyuan
中科院分区：
文献类型：
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作者：
Deng, Xiaomin;Zhou, Shiyi;He, Guangyuan
钙调神经磷酸酶B样蛋白相互作用蛋白激酶（CIPKs）是响应非生物胁迫的Ca 2+信号的组分。在这项工作中，一个新的CIPK基因（TaCIPK 14）的全长cDNA从小麦中分离，并发现有显着的序列相似性OsCIPK 14/15。亚细胞定位分析揭示了TaCIPK 14在整个细胞中的存在。qRT-PCR分析表明，TaCIPK 14在低温条件下或用盐、PEG或外源胁迫相关信号分子（包括阿坝、乙烯和H2 O2）处理时上调。在低温和盐胁迫下，过量表达TaCIPK 14的转基因烟草表现出较高的叶绿素和糖含量，较高的过氧化氢酶活性，较低的过氧化氢和丙二醛含量，较低的离子渗漏。此外，过量表达还提高了盐胁迫下转基因株系的种子发芽率、根伸长和降低了Na+含量。在胁迫条件下，与对照相比，在过表达TaCIPK 14的品系中观察到胁迫相关基因的更高表达。综上所述，这些结果表明TaCIPK 14是植物中的非生物胁迫响应基因。
Calcineurin B-like protein-interacting protein kinases (CIPKs) are components of Ca2+ signaling in responses to abiotic stresses. In this work, the full-length cDNA of a novel CIPK gene (TaCIPK14) was isolated from wheat and was found to have significant sequence similarity to OsCIPK14/15. Subcellular localization assay revealed the presence of TaCIPK14 throughout the cell. qRT-PCR analysis showed that TaCIPK14 was upregulated under cold conditions or when treated with salt, PEG or exogenous stresses related signaling molecules including ABA, ethylene and H2O2. Transgenic tobaccos overexpressing TaCIPK14 exhibited higher contents of chlorophyll and sugar, higher catalase activity, while decreased amounts of H2O2 and malondialdehyde, and lesser ion leakage under cold and salt stresses. In addition, overexpression also increased seed germination rate, root elongation and decreased Na+ content in the transgenic lines under salt stress. Higher expression of stress-related genes was observed in lines overexpressing TaCIPK14 compared to controls under stress conditions. In summary, these results suggested that TaCIPK14 is an abiotic stress-responsive gene in plants.