Neurite bridging across micropatterned grooves
Neurite bridging across micropatterned grooves
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DOI：
10.1016/j.biomaterials.2005.06.035
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发表时间：
2006-01-01
期刊：
影响因子：
14
通讯作者：
Hoffman-Kim, D
中科院分区：
文献类型：
--
作者：
Goldner, JS;Bruder, JM;Hoffman-Kim, D
损伤后，再生轴突必须在复杂的三维（3D）微环境中导航。神经突生长的地形学指导已经在体外用含有纳米微米尺度上的微图案特征的培养基质证明。在这项研究中，我们报告的能力，微制造的生物材料，以支持神经突延伸的微图案化的凹槽与功能尺寸的顺序为几十微米，大小相关的生物材料和组织工程支架的设计。采用聚赖氨酸和层粘连蛋白包被的聚二甲基硅氧烷为基底，培养新生大鼠背根神经节（DRG）神经元。在这里，我们描述了一个不寻常的能力，一个亚群的DRG神经元延长神经突跨越槽，没有潜在的坚实的支持。多个参数影响桥接神经突的形成，在以下实验条件下观察到最高数量的桥：每个样品125，000个细胞的细胞密度，50 μ m的槽深，30 μ m的槽宽，和200 μ m的平台宽度。当神经突从沟槽中的神经元延伸出来，接触相邻的高原，将神经元向上拉，使其悬在沟槽上方，然后索马移位到高原，就形成了桥。这些研究对于理解细胞骨架动力学和设计用于3D轴突引导的生物材料具有重要意义。(c)2005爱思唯尔有限公司保留所有权利。
After injury, regenerating axons must navigate complex, three-dimensional (3D) microenvironments. Topographic guidance of neurite outgrowth has been demonstrated in vitro with culture substrates that contain micropatterned features on the nanometermicron scale. In this study we report the ability of microfabricated biomaterials to support neurite extension across micropatterned grooves with feature sizes on the order of tens of microns, sizes relevant to the design of biomaterials and tissue engineering scaffolds. Neonatal rat dorsal root ganglion (DRG) neurons were cultured on grooved substrates of poly(dimethyl siloxane) coated with poly-L-lysine and laminin. Here we describe an unusual capability of a subpopulation of DRG neurons to extend neurites that spanned across the grooves, with no underlying solid support. Multiple parameters influenced the formation of bridging neurites, with the highest numbers of bridges observed under the following experimental conditions: cell density of 125,000 cells per sample, groove depth of 50 mu m, groove width of 30 mu m, and plateau width of 200 mu m. Bridges were formed as neurites extended from a neuron in a groove, contacted adjacent plateaus, pulled the neuron up to become suspended over the groove, and the soma translocated to the plateau. These studies are of interest to understanding cytoskeletal dynamics and designing biomaterials for 3D axon guidance. (c) 2005 Elsevier Ltd. All rights reserved.