Recent advances in the superovulation in cattle
Recent advances in the superovulation in cattle
复制标题
DOI：
10.1051/rnd:2002046
复制
发表时间：
2002-11-01
期刊：
影响因子：
--
通讯作者：
Adams, GP
中科院分区：
文献类型：
--
作者：
Mapletoft, RJ;Steward, KB;Adams, GP
超排卵反应的变异性仍然是牛胚胎移植中最令人沮丧的问题之一。从垂体提取物中去除LH往往会减少反应的变异性，并对涉及使用纯化的猪垂体提取物促卵泡素（R）-V的几项研究进行了综述。牛超数排卵反应变异性的主要来源是促性腺激素治疗开始时卵泡的状态。数据支持在卵泡波出现时开始促性腺激素治疗的益处。结合旨在控制卵泡波动力学的技术，如卵泡消融或雌二醇/孕酮治疗，减少了在卵泡发育的不同阶段治疗奶牛所引起的变异性，同时通过利用内源性募集和选择机制改善了反应。新的方案提供了方便，能够快速启动促性腺激素治疗，并在一个自己指定的时间，而不需要发情检测和不牺牲反应。该方法可以用于以25至30天的间隔重复超刺激供体动物，而不考虑发情检测或发情周期的阶段，并且不损害胚胎产生。
The variability in the superovulatory response continues to be one of the most frustrating problems with embryo transfer in cattle. The removal of LH from pituitary extracts has tended to reduce the variability in response, and several studies involving the use of the purified porcine pituitary extract, Folltropin(R)-V are reviewed. The major source of variability in the superovulatory response in cattle is the status of ovarian follicles at the time of initiation of gonadotrophin treatments. Data support the benefits of initiating gonadotrophin treatments at the time of emergence of a follicular wave. Incorporation of techniques designed to control follicular wave dynamics, such as follicular ablation, or treatment with estradiol/progesterone, have reduced the variability caused by treating cows at different stages of follicular development, and at the same time improved response by taking advantage of endogenous recruitment and selection mechanisms. New protocols offer the convenience of being able to initiate gonadotrophin treatments quickly and at a self-appointed time, without the necessity of estrus detection and without sacrificing response. Methods can be used for repeated superstimulation of donor animals at 25 to 30 day intervals, without regard to estrus detection or stage of the estrous cycle, and without compromising embryo production.