Keratin filament disruption in interphase and mitotic cells--how is it induced?
Keratin filament disruption in interphase and mitotic cells--how is it induced?
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间期和有丝分裂细胞中的角蛋白丝破坏——它是如何诱导的？
DOI：
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发表时间：
1987
影响因子：
6.6
通讯作者：
M. Osborn
中科院分区：
文献类型：
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作者：
H. G. Tölle;K. Weber;M. Osborn
我们研究了角蛋白中间丝在上皮细胞系中的不稳定性，试图了解导致超微结构从10 nm细丝向含有2-3 nm细丝的球状聚集体转变的分子机制。我们的结果表明，不同实验室使用的不同生长条件可能解释了文献中关于有丝分裂角蛋白细丝断裂的部分但不是全部差异。这种破坏不仅与细胞类型有关，而且还依赖于亚克隆，并且可以通过改变生长介质中的NaHCO3浓度在一个实例中进行操作。在一些上皮细胞系的间期细胞中，通过在冷低渗缓冲液中孵育，或当细胞用佛波酯预处理，然后在冷生理盐水中孵育，可以诱导出明显类似于集合体转变的细丝。一种假定的可透析和热稳定的因子存在于培养液中，条件是特定的上皮细胞类型的生长可能需要破坏。角蛋白多肽的磷酸化可能在细丝不稳定中起作用。
We have studied the lability of keratin intermediate filaments in epithelial cell lines to try to understand the molecular mechanism that cause the ultrastructural transition from 10 nm filaments to the ball-like aggregates containing 2 to 3 nm filaments. Our results suggest that different growth conditions used in different laboratories may explain some but not all of the discrepancies in the literature on mitotic keratin filament disruption. Such disruption is not only cell type, but also subclone dependent and can be manipulated in one instance by altering the NaHCO3 concentration of the growth medium. An apparently similar filament to aggregate transition can be induced in interphase cells of some epithelial cell lines by incubation in a cold hypotonic buffer, or when cells are pretreated with phorbol ester and then incubated in cold physiological saline. A putative dialyzable and heat-stable factor present in medium conditioned by the growth of particular epithelial cell types may be required for disruption. Keratin polypeptide phosphorylation may play a role in filament labilization.