GROWTH-FACTORS IN VITREOUS AND SUBRETINAL FLUID CELLS FROM PATIENTS WITH PROLIFERATIVE VITREORETINOPATHY
GROWTH-FACTORS IN VITREOUS AND SUBRETINAL FLUID CELLS FROM PATIENTS WITH PROLIFERATIVE VITREORETINOPATHY
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DOI：
10.1159/000267221
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发表时间：
1993-01-01
影响因子：
2.1
通讯作者：
GASTAUD, P
中科院分区：
文献类型：
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作者：
BAUDOUIN, C;FREDJREYGROBELLET, D;GASTAUD, P
孔源性视网膜脱离患者发生增殖性玻璃体视网膜病变(PVR)的机制尚不清楚。在之前的一项研究中，我们发现在PVR患者手术切除的视网膜前膜中存在各种生长因子。本文对不同发育阶段的PVR患者的玻璃体内和视网膜下液细胞进行了免疫组织化学研究，以寻找4种视网膜色素上皮细胞生长促进因子：酸性成纤维细胞生长因子、表皮生长因子、胰岛素样生长因子-I和转化生长因子-β。玻璃体和视网膜下积液中的结果非常相似。酸性成纤维细胞生长因子在所有玻璃体和视网膜下标本中均可检测到，30%-100%的细胞中有酸性成纤维细胞生长因子。53%的玻璃体内细胞和69%的视网膜下液细胞表达EGF。除1例外，所有玻璃体标本和视网膜下液标本均可见阳性细胞。15个玻璃体标本中有13个含有IGF-I阳性细胞，20个视网膜下液标本中有18个阳性细胞(阳性标本的平均反应率分别为70%和78%)。相反，在15个玻璃体样本中只有8个样本和20个视网膜下样本中的11个样本中发现了转化生长因子-β1的阳性反应。平均反应性细胞百分比分别为30%和50%。提示在PVR过程中，多种生长因子可能参与了视网膜色素上皮细胞的增殖和迁移。然而，它们各自的作用以及它们是单独行动还是协同行动，是仍有待调查的众多问题中的一部分。
Mechanisms accounting for the development of proliferative vitreoretinopathy (PVR) in patients with rhegmatogenous retinal detachment remain poorly understood. In a previous study, we found the presence of various growth factors in preretinal membranes that were surgically removed from patients with PVR. The present immunohistological study was undertaken in intravitreal and subretinal fluid cells from patients suffering from PVR in various stages of development, in order to seek the presence of 4 growth-promoting factors for retinal pigment epithelial cells: acidic fibroblast growth factor (FGF), epidermal growth factor (EGF), insulin-like growth factor type I (IGF-I) and transforming growth factor-beta (TGF-beta). Results were quite similar in vitreous and subretinal fluid. Acidic FGF was found in all vitreous and subretinal specimens, in 30-100% of the examined cells. Immunoreactivity for EGF could be found in 53% of intravitreal cells and 69% of subretinal fluid cells. Positive cells were seen in all vitreous specimens and in all but 1 of the subretinal fluid specimens. IGF-I-containing cells were present in 13 of 15 vitreous specimens and in 18 of 20 subretinal fluid samples (mean percentages of reactivity in positive specimens 70% and 78%, respectively). In contrast, TGF-beta1 reactivity was found in only 8 of 15 vitreous specimens and in 11 of 20 subretinal samples. Mean percentages of reactive cells were 30% and 50%, respectively. These results suggest that several growth factors could be involved in the proliferation and migration of retinal pigment epithelial cells during the course of PVR. Their respective role and whether they act solely or synergistically are, however, some of the numerous questions that remain to be investigated.