REGION-X, THE CELL-WALL-ATTACHMENT PART OF STAPHYLOCOCCAL PROTEIN-A
REGION-X, THE CELL-WALL-ATTACHMENT PART OF STAPHYLOCOCCAL PROTEIN-A
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DOI：
10.1111/j.1432-1033.1984.tb07931.x
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
SJODAHL, J
中科院分区：
文献类型：
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作者：
GUSS, B;UHLEN, M;SJODAHL, J
测定了葡萄球菌A蛋白X区的序列。假设该区域跨越金黄色葡萄球菌细胞壁并负责与肽聚糖结合。确定了2株显示细胞壁结合蛋白A的菌株的X区的一级氨基酸序列。科万I和8325-4。与通过相应基因的核苷酸测序完全分析的8325-4材料的序列相反，科万I材料的序列测定是部分的，并且是通过Edman降解进行的。该区域由2个结构不同的结构域组成，高度重复区域（Xr），具有重复的八肽结构。12倍，和一个C-末端结构域（Xc）具有独特的序列。2株之间的比较揭示了八肽结构之间的高度相互同源性以及高度内部同源性。8个氨基酸中的6个在两种蛋白质的整个Xr区域中重复该结构是相同的，另外2个以相当规则的模式变化。
The sequence of region X of staphylococcal protein A was determined. It is hypothesized that this region spans the Staphylococcus aureus cell wall and is responsible for the binding to the peptidoglycan. The primary amino acid sequence of region X was determined for 2 strains exhibiting cell-wall-bound protein A. Cowan I and 8325-4. The sequence determination of the Cowan I material is partial and was performed by Edman degradation, in contrast to the sequence of the 8325-4 material which was completely analyzed by nucleotide sequencing of the corresponding gene. The region consists of 2 structurally different domains, a highly repetitive region (Xr), with an octapeptide structure repeated .apprx. 12 times, and a C-terminal domain (Xc) with an unique sequence. A comparison between the 2 strains reveals a high mutual homology as well as a high internal homology between the octapeptide structures. Six of 8 amino acids are identical in the repetition of this structure throughout region Xr in both proteins and the other 2 are changed in a rather regular pattern.