A lipid transfer protein gene BG-14 is differentially regulated by abiotic stress, ABA, anisomycin, and sphingosine in bromegrass (Bromus inermis).
A lipid transfer protein gene BG-14 is differentially regulated by abiotic stress, ABA, anisomycin, and sphingosine in bromegrass (Bromus inermis).
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DOI：
10.1078/0176-1617-01259
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发表时间：
2004
影响因子：
4.3
通讯作者：
Guohai Wu;A. Robertson;Xunjia Liu;P. Zheng;R. Wilen;N. T. Nesbitt;L. Gusta
中科院分区：
文献类型：
--
作者：
Guohai Wu;A. Robertson;Xunjia Liu;P. Zheng;R. Wilen;N. T. Nesbitt;L. Gusta
本研究旨在研究无芒雀麦细胞和幼苗在非生物胁迫、脱落酸(ABA)、山奈素和鞘氨醇胁迫下脂转移蛋白基因(LTP)的表达。从无芒雀麦悬浮细胞中分离的全长cDNA克隆BG-14编码一个124个氨基酸的多肽，具有典型的LTP特征，如半胱氨酸残基的保守排列。在冷驯化活跃阶段，LTP的表达上调，而在冷驯化的末期，LTP转录水平下降到驯化前的水平。严重的干旱胁迫诱导了LTP基因的表达，但在复水后3d，LTP的表达增加了一倍。温度和热激持续时间都影响LTP的诱导，但温度是主要影响因素。盐处理在15min内刺激LTP mRNA的积累，并在盐胁迫期间保持在较高的水平。最有趣的是，Northern杂交显示，在悬浮细胞培养中，LTP不仅被ABA迅速诱导，而且还被山奈素和鞘氨醇诱导。在三种化学物质中，ABA诱导LTP表达的反应最快和最高，耐寒性最强，而鞘氨醇对LTP表达和耐寒性的活性最低。
The objective was to investigate the expression of a lipid transfer protein gene (LTP) both in bromegrass (Bromus inermis) cells and seedlings after exposure to abiotic stresses, abscisic acid (ABA), anisomycin, and sphingosine. A full-length cDNA clone BG-14 isolated from bromegrass suspension cell culture encodes a polypeptide of 124 amino acids with typical LTP characteristics, such as a conserved arrangement of cysteine residues. During active stages of cold acclimation LTP expression was up-regulated, whereas at the final stage of cold acclimation LTP transcript level declined to pre-acclimation level. A severe drought stress induced the LTP gene; yet, LTP expression doubled 3 d after re-hydration. Both temperature and heat shock duration influence LTP induction; however temperature is the primary factor. Treatment with NaCl stimulated accumulation of LTP mRNA within 15 min and the transcripts remained at elevated levels for the duration of the salinity stress. Most interestingly, Northern blots showed LTP was rapidly induced not only by ABA, but also by anisomycin and sphingosine in suspension cell cultures. Of the three chemicals, ABA induced the most rapid and highest response in LTP expression as well as highest freezing tolerance, whereas sphingosine was the least active for both LTP expression and freezing tolerance.