Structural basis of transcription by bacterial and eukaryotic RNA polymerases.
Structural basis of transcription by bacterial and eukaryotic RNA polymerases.
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细菌和真核 RNA 聚合酶转录的结构基础。
DOI：
10.1016/j.sbi.2011.11.006
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
et al.
中科院分区：
文献类型：
--
作者：
Sekine;S.;et al.
DNA依赖性RNA聚合酶（RNAP）负责细胞基因转录。虽然对原核和真核RNAP的晶体学研究已经阐明了基本的RNAP结构，但在转录起始、延伸和终止过程中许多重要事件的结构细节仍然在很大程度上未知。最近对细菌RNAP和酵母RNAP II的晶体学研究揭示了与正常转录复合物不同的RNAP结构状态，以及转录因子功能的基础，推进了我们对转录的理解。这些研究突出了细菌和真核生物转录机制在许多基本方面的意想不到的相似性。细菌和真核生物的转录系统之间也存在显著差异，为未来的研究提供了方向。
DNA-dependent RNA polymerase (RNAP) is responsible for cellular gene transcription. Although crystallographic studies on prokaryotic and eukaryotic RNAPs have elucidated the basic RNAP architectures, the structural details of many essential events during transcription initiation, elongation, and termination are still largely unknown. Recent crystallographic studies on a bacterial RNAP and yeast RNAP II have revealed different RNAP structural states from that of the normal transcribing complex, as well as the basis of transcription factor functions, advancing our understanding of transcription. These studies have highlighted unexpected similarities in many fundamental aspects of transcription mechanisms between the bacterial and eukaryotic transcription machineries. Remarkable differences also exist between the bacterial and eukaryotic transcription systems, suggesting directions for future studies.