Flagellar elongation and shortening in chlamydomonas. II. Re-utilization of flagellar proteins.
Flagellar elongation and shortening in chlamydomonas. II. Re-utilization of flagellar proteins.
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DOI：
10.1083/jcb.47.3.777
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发表时间：
1970-12
影响因子：
7.8
通讯作者：
Rosenbaum, J L
中科院分区：
文献类型：
--
作者：
Coyne, B;Rosenbaum, J L
《细胞生物学杂志》第47卷。1970年777-781777页的长度大致相等，因此两个鞭毛通过减速动力学一起伸长。在没有蛋白质合成的情况下，事件的模式类似于不受限制的长零细胞：完整的鞭毛仍然缩短，被截断的鞭毛延长，当它们达到相同的长度时，它们一起伸长。这里特别有趣的是观察到，在没有蛋白质合成的情况下，长零细胞中形成的新鞭毛的总长度比去掉两个鞭毛的受抑细胞中形成的新鞭毛的总长度要大得多。这一结果表明，放线菌胺抑制的Long Zero细胞中额外的鞭毛生长可能是通过重复利用缩短鞭毛中的鞭毛蛋白来实现的。如果是这样的话，对于在放线菌亚胺存在下的长零细胞，完整鞭毛的初始长度越大，随后通过再生获得的鞭毛长度就越大。此外，如果鞭毛前体蛋白实际上是在两个鞭毛之间平均分配的，那么对于一个长零细胞中最初存在的每一个额外的完整鞭毛微米，每个鞭毛的最终长度应该增加0.5微米。在放线菌亚胺存在下，对Long-Zero细胞不同初始长度的单个细胞的最终鞭毛长度的测量结果与这一预测非常一致，并支持从缩短的鞭毛中提取的鞭毛蛋白被重新用于随后的鞭毛再生的结论。
TILE JOURNAL OF CELL BIOLOGY±VOLUME 47. 1970. pages 777-781 777 approximately equal length, whereupon the two flagella elongate together by deceleratory kinetics. In the absence of protein synthesis, the pattern of events is similar to that occurring in uninhibited long-zero cells: The intact flagellum still shortens and the amputated flagellum elongates, and when they reach the same length they elongate together. Of particular interest here was the observation that in the absence of protein synthesis the total length of new flagellum formed in the long-zero cells was considerably greater than that formed in inhibited cells with both flagella removed. This result suggested that the additional flagellar growth in cycloheximide-inhibited longzero cells might be occurring by re-utilization of flagellar proteins from the shortening flagellum. If this were so, then, for long-zero cells in the presence of cycloheximide, the greater the initial length of the intact flagellum, the greater should be the flagellar length subsequently attained by regeneration. Moreover, for each additional micron of intact flagellum initially present in a longzero cell, there should be one-half micron added to the final length of each flagellum if, in fact, the flagellar precursor protein is being divided equally between the two flagella. Measurements reported here of the final flagellar lengths of single cells for a variety of initial lengths of the intact flagellum of long-zero cells in the presence of cycloheximide are in excellent agreement with this prediction, and support the conclusion that flagellar proteins from the shortening flagellum are re-utilized for the subsequent flagellar regeneration.