In situ expression of cagA and risk of gastroduodenal disease in Helicobacter pylori-infected children.
In situ expression of cagA and risk of gastroduodenal disease in Helicobacter pylori-infected children.
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幽门螺杆菌感染儿童中 cagA 的原位表达与胃十二指肠疾病的风险。
DOI：
10.1097/mpg.0b013e3181bab326
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发表时间：
2010
影响因子：
2.9
通讯作者：
Dubois,Andre
中科院分区：
文献类型：
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作者：
Rick,JamesR;Goldman,Matthew;Semino-Mora,Cristina;Liu,Hui;Olsen,Cara;Rueda-Pedraza,Eugenia;Sullivan,Carolyn;Dubois,Andre
结果：内镜检查正常（n= 14）或显示结节（n= 18），胃溃疡（n= 8）或十二指肠溃疡（n= 11）。幽门螺杆菌分别存在于7，18，6和10名儿童。溃疡组16SrRNA和cagA的表达明显高于对照组。结论：幽门螺杆菌感染的儿童消化性溃疡中cagA原位表达增加，cagA原位表达可能参与了儿童消化性溃疡的发病过程。cagA原位表达的测定补充了传统的分离和单菌落分离株的体外检测。
Results:Endoscopy was either normal (n= 14) or demonstrated nodularity (n= 18), gastric ulcer (n= 8) or duodenal ulcer (n= 11). H pylori was present in 7, 18, 6, and 10 children, respectively. Expression of 16S rRNA and cagA were significantly higher in children with ulcer compared with normal children. The fraction of H pylori bacteria expressing cagA in situ was higher in children with ulcer compared to those with endoscopic nodularity (P< 0.05).Conclusions:Thus, cagA in situ expression is increased in H pylori–infected children with peptic ulcers and may play a role in the pathogenesis of peptic ulcer disease during childhood. Determination of in situ expression of cagA complements traditional isolation and in vitro testing of single-colony isolates.