Targeting coat protein II complex genes via RNA interference inhibits female adult feeding and reproductive development in the cabbage beetle Colaphellus bowringi
Targeting coat protein II complex genes via RNA interference inhibits female adult feeding and reproductive development in the cabbage beetle Colaphellus bowringi
复制标题
通过RNA干扰靶向外壳蛋白II复合物基因抑制卷心菜甲虫Colaphellus Bowringi雌性成虫的摄食和生殖发育
DOI：
10.1002/ps.6836
复制
发表时间：
2022
影响因子：
4.1
通讯作者：
Xiao‐Ping Wang
中科院分区：
文献类型：
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作者：
Zhong Tian;Shuang Guo;Fen Zhu;Wen Liu;Xiao‐Ping Wang
背景甘蓝小卷蛾是亚洲十字花科蔬菜的一种极具破坏性的害虫。这种甲虫主要由合成化学农药控制，在食物上留下农药残留，对人类健康构成重大隐患。在初步研究的基础上，我们推测外壳蛋白II(COPII)复合体是一种从内质网输出货物分子的初级包膜囊泡，是防治茶尺蛾的新靶标。结果本研究探讨了利用RNA干扰(RNAi)干扰COPII是否影响茶尺蛾成虫的生长发育。结果表明，5个COPII装配基因Sar1、Sec23、SEC24、Sec13和Sec31在成虫多个组织中均有表达。注射针对每个基因的双链RNA(DsRNA)可诱导约55-99%的高RNAi效率，并显著抑制卵黄沉积和卵巢生长。此外，Sar1、Sec23和Sec24基因的敲除抑制了食性，使死亡率分别增加到26.67%、46.67%和42.22%。这部分是由于胰岛素/mTOR相关的营养通路下调所致。结果表明，沉默负责COPII复合体组装的5个基因中的任何一个都抑制了保幼激素和蜕皮激素信号通路，这表明囊泡运输在保龄虫雌虫的内分泌调节中起着至关重要的作用。结论本研究表明COPII复合体可能是一个有希望的RNAi靶标，用于治理保龄虫，从而减少我们对化学农药防治害虫的依赖。©2022化学工业学会。
BACKGROUNDThe cabbage beetleColaphellus bowringiis a highly destructive cruciferous vegetable pest in Asia. This beetle is predominantly controlled by synthetic chemical pesticides, which leave pesticide residues on food and constitute a major hidden danger to human health. Based on preliminary research, we hypothesized that the coat protein II (COPII) complex, a primary coated vesicle that exports cargo molecules from the endoplasmic reticulum, is a promising novel target for the control ofColaphellus bowringi.RESULTSThis study investigated whether disrupting COPII using RNA interference (RNAi) affects the growth and development ofColaphellus bowringiadults. The results showed that five COPII assembly genes,Sar1,Sec23,Sec24,Sec13, andSec31, were uniformly expressed in multiple tissues of adult femaleColaphellus bowringi. Injecting double‐stranded RNA (dsRNA) against each gene induced a high RNAi efficiency by approximately 55–99%, and considerably inhibited yolk deposition and ovarian growth. Moreover, knockdown ofSar1,Sec23andSec24suppressed feeding and increased mortality to 26.67%, 46.67%, and 42.22%, respectively. This was partially due to the down‐regulation of insulin/mTOR‐associated nutritional pathways. The results indicate that silencing any of the five genes responsible for COPII complex assembly represses Juvenile hormone and ecdysone signaling pathways, suggesting that vesicle transport plays a vital role in the endocrine regulation ofColaphellus bowringifemales.CONCLUSIONThis study suggests that the COPII complex could be a promising RNAi target for the management ofColaphellus bowringi, which would reduce our dependence on chemical pesticides for pest control. © 2022 Society of Chemical Industry.