Aspergillus verrucosus sp. nov., a xerophilic species isolated from house dust and honey in Japan
Aspergillus verrucosus sp. nov., a xerophilic species isolated from house dust and honey in Japan
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疣状曲霉 sp.
DOI：
10.1099/ijsem.0.005727
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发表时间：
2023
影响因子：
2.8
通讯作者：
Dai Hirose
中科院分区：
文献类型：
--
作者：
Ryo Hagiuda;Tadashi Itagaki;Dai Hirose
从日本的屋尘和蜂蜜中分离到3株嗜旱菌。基于四个区域（内转录间隔区rDNA、钙调蛋白、β-微管蛋白和RNA聚合酶II第二大亚基）的组合数据集的分子系统发育分析显示，这些菌株形成了一个独立的谱系，与在限制性部分中分类的嗜盐曲霉（Aspergillus halophilicus）是姐妹。形态学比较表明，菌株不同于A。嗜盐菌在三个方面：（i）闭囊壳的大小，以及子囊孢子的表面结构和大小，（ii）在Harrold琼脂和dichloran 18%甘油琼脂上生长的能力 ，和（iii）在马铃薯葡萄糖琼脂+20%NaCl上缺乏分生孢子梗形成 。这些菌株与已知的革兰氏阴性菌限制种（SectionRestrictispecies）有明显的区别。因此，我们认为它是一个新种，并建议命名为Aspergillus verrucosussp。（NBRC 115547T）。
Three strains of a xerophilicAspergillusspecies were isolated from house dust and honey in Japan. A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit) revealed that the strains formed an independent lineage, sister toAspergillus halophilicusclassified in sectionRestricti. Morphological comparisons show that the strains differ fromA. halophilicusin three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold’s agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl. These strains could be clearly distinguished from all knownAspergillussectionRestrictispecies. Therefore, we consider it to be a novel species and propose the nameAspergillus verrucosussp. nov. (NBRC 115547T).