Diurnal triglyceride profiles: a novel approach to study triglyceride changes
Diurnal triglyceride profiles: a novel approach to study triglyceride changes
复制标题
DOI：
10.1016/s0021-9150(00)00554-2
复制
发表时间：
2001-03-01
期刊：
影响因子：
5.3
通讯作者：
Erkelens, DW
中科院分区：
文献类型：
--
作者：
Cabezas, MC;Halkes, CJM;Erkelens, DW
空腹血浆甘油三酯 (TG) 显示出较高的个体内变异性，因此，在研究 TG 代谢时，需要重复测量和替代方法。为了寻找研究 TG 变化的新方法，我们评估了测定动态毛细血管 TG 的可行性。此外，还确定了健康受试者中众所周知的 TG 代谢特征（例如性别差异）。在 18 名空腹血浆 TG 范围广泛的受试者中，将标准化口腔脂肪负荷测试 (50 g m(-2)) 的结果与其每日毛细血管 TG 曲线进行比较，该曲线在 3 个不同的日子、每天在门诊诊所测量 6 次。每日 TG 曲线计算为毛细管 TG 曲线下面积 (TGc-AUC) 和增量面积 (dTGc-AUC)。急性口腔脂肪负荷后血浆 TG 清除率与每日 TGc-AUC 密切相关（r = 0.77；P < 0.01）。此外，与空腹血浆正常的受试者相比，高甘油三酯血症受试者（血浆TG > 2.0 mmol l(-1)）的日间甘油三酯血症较高（49.83 +/- 15.37 h mmol l(-1)），血浆TG对口腔脂肪负荷的反应也较高（42.10 +/- 15.37 h mmol l(-1)）。 TG（分别为29.83 +/- 11.75 h mmol l(-1) (P < 0.05) 和20.75 +/- 5.89 h mmol l(-1) (P < 0.01)）。在一项观察性研究中，106 名志愿者（54 名女性和 52 名男性）测量了毛细血管甘油三酯。在研究开始时记录食物摄入量并抽取一次空腹血。通过人体测量参数和身体阻抗评估身体成分。每日甘油三酯血症的重复测量往往比空腹毛细血管甘油三酯的变化更小（整个组和男性的平均变异系数分别为 15.1%（范围：0.60-45.9%）和 24.9%（范围：1.44-72.7%）；P = 0.09）（18.6%（0.60-45.9%）和24.0% 分别为（1.4-58.2%）； P = 0.07）。女性的平均每日TGc-AUC和dTGc-AUC（分别为16.50+/-4.85和1.82+/-3.46hmmoll(-1)）低于男性（23.44+/-6.50和6.93+/-4.67hmmoll(-1)；均P<0.001）。由于所有营养素的摄入量较低，女性每日总能量摄入量（8911 +/- 1905 kJ）低于男性（11042 +/- 2604 kJ，P < 0.001）。在女性中，雌激素状态显着决定了毛细血管甘油三酯的分布。以 TGc-AUC 作为因变量对女性和男性进行的逐步多元回归分析表明，最佳预测因素是空腹毛细血管 TG、性别、收缩压和平均每日能量摄入量，解释了 72% 的变异。甘油三酯血症增量最好通过性别、平均每日蛋白质摄入量和收缩压来描述，解释了 42% 的变异。每日毛细血管TG曲线可用于估计个体在白天所承受的潜在致动脉粥样硬化颗粒的每日总负荷，而无需进行代谢病房研究。 (C) 2001 Elsevier Science Ireland Ltd. 保留所有权利。
Fasting plasma triglycerides (TG) show a high intra-individual variability, and therefore, repeated measurements and alternative methodology are necessary when studying TG metabolism. In search for novel approaches to study TG changes, we evaluated the feasibility of determining ambulatory capillary TG. In addition, well-known characteristics (e.g. gender differences) of TG metabolism in healthy subjects were determined. In 18 subjects with a wide range of fasting plasma TG, the results of standardised oral fat loading tests (50 g m(-2)) were compared to their diurnal capillary TG profiles, measured on 3 different days, six times each day in an out-patient clinic setting. The diurnal TG-profile was calculated as area under the capillary TG curve (TGc-AUC) and as incremental area (dTGc-AUC). Clearance of plasma TG after the acute oral fat load correlated well with the diurnal TGc-AUC (r = 0.77; P < 0.01). In addition, hypertriglyceridemic subjects (plasma TG > 2.0 mmol l(-1)) had a higher diurnal triglyceridemia (49.83 +/- 15.37 h mmol l(-1)) as well as a higher response of plasma TG to the oral fat load (42.10 +/- 15.37 h mmol l(-1)), than the subjects with normal fasting plasma TG (29.83 +/- 11.75 h mmol l(-1) (P < 0.05) and 20.75 +/- 5.89 h mmol l(-1) (P < 0.01), respectively). In an observational study, 106 volunteers (54 females and 52 males) measured capillary triglycerides. Food intake was recorded and fasting blood was drawn once at the start of the study. Body composition was assessed by anthropometric parameters and body-impedance. Repeated measurements of diurnal triglyceridemia tended to be less variable than fasting capillary triglycerides (mean coefficients of variation 15.1% (range: 0.60-45.9%) and 24.9% (range: 1.44-72.7%), respectively; P = 0.09) for the whole group and in males (18.6% (0.60-45.9%) and 24.0% (1.4-58.2%), respectively; P = 0.07). The mean diurnal TGc-AUC and dTGc-AUC were lower in females (16.50 +/- 4.85 and 1.82 +/- 3.46 h mmol l(-1), respectively) than in males (23.44 +/- 6.50 and 6.93 +/- 4.67 h mmol l(-1); P < 0.001 for each). The total daily energy intake was lower in females (8911 +/- 1905 kJ) than in males (11042 +/- 2604 kJ, P < 0.001) because of a lower intake of all nutrients. In females, estrogen status determined significantly the capillary TG profiles. Stepwise multiple regression analysis for females and males, with TGc-AUC as the dependent variable, showed that the best predictors were fasting capillary TG, gender, systolic blood pressure and mean daily energy intake, explaining 72% of the variation. Incremental triglyceridemia was best described by gender, mean daily protein intake and systolic blood pressure, explaining 42% of the variation. Diurnal capillary TG profiles may be used to estimate the total daily load of potential atherogenic particles to which individuals are subjected during the day without the need for metabolic ward studies. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.