A novel fluorescent probe for the detection of nitric oxide in vitro and in vivo
A novel fluorescent probe for the detection of nitric oxide in vitro and in vivo
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一种用于体外和体内检测一氧化氮的新型荧光探针
DOI：
10.1016/j.freeradbiomed.2008.08.016
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发表时间：
2008-11-15
影响因子：
7.4
通讯作者：
Zhang, Junfeng
中科院分区：
文献类型：
--
作者：
Ouyang, Jie;Hong, Hao;Zhang, Junfeng
一氧化氮 (NO) 体外和体内荧光成像对于加深我们对一氧化氮在生物学和医学中的作用的理解至关重要。目前的探针，例如二氨基荧光素，依赖于与氧化NO产物的反应，而不是直接与一氧化氮的反应，这限制了它们的适用性。在这里，我们报告了通过将高荧光化学物质 4-methoxy-2-(1H-naphtho[2,3-d]imidazol-2-yl)phen (MNIP) 与 Cu(II) 配位形成一氧化氮成像探针。配位化合物 MNIP-Cu 与一氧化氮快速、特异地反应，生成可在体外和体内使用的蓝色荧光产物。在本研究中，MNIP-Cu 用于揭示脂多糖 (I-PS) 激活的巨噬细胞（Raw 264.7 细胞）中诱导型一氧化氮合酶和内皮细胞 (HUVEC) 中内皮一氧化氮合酶产生的一氧化氮。 MNIP-Cu 还用于评估小鼠急性肝损伤诱导的 LPS 和 n-半乳糖胺模型中一氧化氮合成的分布。结果表明，MNIP-Cu可以作为一种新型的一氧化氮荧光探针，在生物医学研究中具有许多潜在的应用。 (C) 2008 Elsevier Inc. 保留所有权利。
Fluorescence imaging, of nitric oxide (NO) in vitro and in vivo is essential to developing Our understanding of the role of nitric oxide in biology and medicine. Current probes Such as diaminofluorescein depend on reactions with oxidized NO products, but not with nitric oxide directly, and this limits their applicability. Here we report the formation of an imaging probe for nitric oxide by coordinating the highly fluorescent chemical 4-methoxy-2-(1H-naphtho[2,3-d]imidazol-2-yl)phenol (MNIP) with Cu(II). The coordination compound MNIP-Cu reacts rapidly and specifically with nitric oxide to generate a product with blue fluorescence that can be used in vitro and in vivo. In the present study MNIP-Cu was used to reveal nitric oxide produced by inducible nitric oxide synthase in lipopolysaccharide (I-PS)activated macrophages (Raw 264.7 cells) and by endothelial nitric oxide synthase in endothelial cells (HUVEC). MNIP-Cu Was also Used to evaluate the distribution of nitric oxide synthesis in a model of acute liver injury induced LPS and n-galactosamine in mice. The results demonstrate that MNIP-Cu can act as a novel fluorescent probe for nitric oxide and has many potential applications in biomedical research. (C) 2008 Elsevier Inc. All rights reserved.