SpeedyGenesXL: an Automated, High-Throughput Platform for the Preparation of Bespoke Ultralarge Variant Libraries for Directed Evolution.
SpeedyGenesXL: an Automated, High-Throughput Platform for the Preparation of Bespoke Ultralarge Variant Libraries for Directed Evolution.
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SpeedyGenesXL：一个自动化的高通量平台，用于为定向进化准备定制的超大型变体文库。
DOI：
10.1007/978-1-0716-2152-3_5
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Sadler JC
中科院分区：
文献类型：
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作者：
Sadler JC
蛋白质的定向进化是一种针对特定应用定制生物催化剂的高效策略，并且能够进行工程改进，例如 Askcat、热稳定性和有机溶剂耐受性。人们认识到，需要大型和系统的文库来有效地导航蛋白质庞大而崎岖的序列景观，但它们的准备工作并不简单，而且商业文库的成本极高。为了解决这个问题，我们开发了 SpeedyGenesXL，这是一个自动化的高通量平台，用于基于 SpeedyGenes 方法生产野生型基因、布尔 OR、组合或组合 OR 型文库。总之，这为库合成提供了一个灵活的平台，能够同时生成许多不同的定制、多样化的库。
Directed evolution of proteins is a highly effective strategy for tailoring biocatalysts to a particular application, and is capable of engineering improvements such askcat,thermostability and organic solvent tolerance. It is recognized that large and systematic libraries are required to navigate a protein’s vast and rugged sequence landscape effectively, yet their preparation is nontrivial and commercial libraries are extremely costly. To address this, we have developed SpeedyGenesXL, an automated, high-throughput platform for the production of wild-type genes, Boolean OR, combinatorial, or combinatorial-OR-type libraries based on the SpeedyGenes methodology. Together this offers a flexible platform for library synthesis, capable of generating many different bespoke, diverse libraries simultaneously.