Ultrasound-targeted microbubble destruction enhances naked plasmid DNA transfection in rabbit Achilles tendons in vivo
Ultrasound-targeted microbubble destruction enhances naked plasmid DNA transfection in rabbit Achilles tendons in vivo
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DOI：
10.1038/gt.2011.165
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发表时间：
2011-10
期刊：
影响因子：
5.1
通讯作者：
Li Qiu;Ling-yan Zhang;Lei Wang;Yong Jiang;Yan Luo;Yu-Lan Peng;Ling Lin
中科院分区：
文献类型：
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作者：
Li Qiu;Ling-yan Zhang;Lei Wang;Yong Jiang;Yan Luo;Yu-Lan Peng;Ling Lin
本研究旨在探讨超声靶向微泡破坏（UTMD）增加该基因在肌腱中转染的可能性，并寻找最合适的转染条件。采用不同给药途径将微泡与增强绿色荧光蛋白（EGFP）质粒混合注入兔跟腱，并在不同超声条件下对跟腱进行超声脉冲，以确定最适宜的给药条件。然后将家兔分为四组：(1)超声+微泡+质粒；(2)超声+质粒；(3)微泡+质粒；(4)仅质粒。观察EGFP在肌腱和其他组织中的表达情况，以及对肌腱和副肌腱的损伤情况。结果表明，输出强度为2 W cm−2、占空比为20%、脉冲时间为10 min的超声脉冲能显著提高EGFP在肌腱中的表达。局部注射是较好的给药途径。四组中，第1组EGFP表达量高于其他各组。EGFP在第7天表达量最高，随后逐渐降低，并持续56 d以上。其他组织中未见EGFP表达。UTMD未造成明显损伤。在合适的条件下，UTMD作为一种安全有效的肌腱损伤基因转染疗法是可行的。
The study was to investigate the probability of increasing the transfection of the gene in tendons by ultrasound-targeted microbubble destruction (UTMD), and to search for the most suitable transfection conditions. A mixture of microbubbles and enhanced green fluorescent protein (EGFP) plasmids was injected into rabbit Achilles tendons by different administration routes and the tendons were ultrasound pulse by different ultrasonic conditions in order to determine the most appropriate conditions. Then, the rabbits were divided into four groups:(1) ultrasound+ microbubbles+ plasmid;(2) ultrasound+ plasmid;(3) microbubble+ plasmid;(4) plasmid only. EGFP expression in the tendons and other tissues, and the damage to tendon and paratenon were all observed. The results showed that EGFP expression in the tendon was higher by ultrasound pulse with 2 W cm− 2 of output intensity and a 20% duty cycle for 10 min. Local injection was determined to be the better administration route. Among the four groups, EGFP expression in Group 1 was higher than that in other groups. EGFP expression was highest on seventh day, then it gradually decrease over time, and lasted more than 56 days. EGFP expression was not found in other tissues. There was no obvious injury caused by UTMD. Under suitable conditions, it is feasible to use UTMD as a safe and effective gene transfection therapy for tendon injuries.