GLIADIN INDUCED CHANGES IN THE EXPRESSION OF MHC-CLASS-II ANTIGENS BY HUMAN SMALL INTESTINAL EPITHELIUM - ORGAN-CULTURE STUDIES WITH CELIAC-DISEASE MUCOSA
GLIADIN INDUCED CHANGES IN THE EXPRESSION OF MHC-CLASS-II ANTIGENS BY HUMAN SMALL INTESTINAL EPITHELIUM - ORGAN-CULTURE STUDIES WITH CELIAC-DISEASE MUCOSA
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DOI：
10.1136/gut.33.4.472
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发表时间：
1992-04-01
期刊：
影响因子：
24.5
通讯作者：
AURICCHIO, S
中科院分区：
文献类型：
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作者：
FAIS, S;MAIURI, L;AURICCHIO, S
来自16名治疗的乳糜泻患者的空肠活检和9个对照组的麦芽胶质素培养和没有消化性的特里蛋白消化。还进行了具有玉米脯氨酸蛋白的消化性植物摘要的培养物。基线和培养的粘膜的冷冻切片用抗HLA-DR单克隆抗体的免疫荧光染色。在培养之前，来自两个对照和处理过的乳糜泻的绒毛上皮表达了DR分子，而隐窝上皮则没有。当用神经胶质素培养来自处理过的乳糜泻的活检时，在14个培养物中的8个中，八个中的八个中，DR的表达得到了增强，而在14种培养物中，DR的表达在绒毛上皮中减少或不存在。在对照文化中没有观察到任何变化。我们得出的结论是，麦醇溶蛋白能够在体外培养的乳糜泻粘膜上诱导HLA-DR，从而提供间接证据，证明麦硫二可以激活小肠粘膜中细胞介导的免疫机制。该模型可以证明可用于识别麦芽胶蛋白和相关脯氨酸的免疫原性序列。
Jejunal biopsies from 16 treated coeliac disease patients and from nine controls were cultured with and without a peptic-tryptic digest of gliadin. Cultures with a peptic-tryptic digest of maize prolamins were also undertaken. Frozen sections of baseline and cultured mucosa were stained by immunofluorescence with an anti-HLA-DR monoclonal antibody. Before culture the villous epithelium from both controls and treated coeliac disease expressed DR molecules while the crypt epithelium did not. When biopsies from treated coeliac disease were cultured with gliadin the expression of DR was enhanced in the crypt epithelium in eight of 14 cultures and in 11 of 14 was reduced or absent on the villous epithelium. No change was observed in control cultures. We conclude that gliadin is capable of inducing HLA-DR on the crypt epithelium of in vitro cultured coeliac disease mucosa, providing indirect evidence that gliadin may activate cell mediated immune mechanisms within the small bowel mucosa. This model could prove useful in identifying the immunogenic sequence(s) of gliadins and related prolamines.