Clonal expansion of multiphenotypic Epstein-Barr virus-infected lymphocytes in chronic active Epstein-Barr virus infection
Clonal expansion of multiphenotypic Epstein-Barr virus-infected lymphocytes in chronic active Epstein-Barr virus infection
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DOI：
10.1016/j.mehy.2004.03.012
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发表时间：
2004-01-01
影响因子：
4.7
通讯作者：
Kobayashi, K
中科院分区：
文献类型：
--
作者：
Endo, R;Yoshioka, M;Kobayashi, K
慢性活动性EB病毒（EBV）感染是一种克隆性非肿瘤性淋巴组织增生性疾病。然而，一些报告的情况下，EBV感染的淋巴细胞的多表型扩增引起的问题，EBV如何感染多表型淋巴细胞和慢性活动性EBV感染是否是一个真正的单克隆淋巴细胞增生性疾病。我们报告两例慢性活动性EB病毒感染的患者，他们表现为多表型EB病毒感染的淋巴细胞扩增。通过聚合酶链反应（PCR），在一名患者的胸水中的CD 4（+）和CD 8（+）T细胞和B细胞中检测到EBV DNA，并且在另一名患者的颈部淋巴结中的T和B细胞中检测到EBV DNA。尽管实时PCR显示，在来自胸膜液的CD4（+）和CD8（+）T细胞中有同样高的EBV基因组载量，但与EBV基因组末端重复序列的Southern印迹杂交显示，在来自患者的三个组织样本中有一条相同分子量的条带。结果表明，同一克隆的EBV感染后，CD4（+）和CD8（+）T细胞出现双表型扩增。此外，亚硫酸氢盐PCR分析显示，无论其细胞群体如何，这两名患者的Cp区域均处于高甲基化状态。多表型EBV感染的淋巴细胞的克隆性和扩增之间存在差异。我们推测未分化成T和B细胞祖细胞的淋巴祖细胞被EBV感染，导致EBV感染的多表型细胞的克隆扩增。(C)2004爱思唯尔有限公司保留所有权利。
Chronic active Epstein-Barr virus (EBV) infection has been recognized as clonal non-neoplastic lymphoproliferative diseases. However, some reports of cases with a multiphenotypic expansion of EBV-infected lymphocytes give rise to questions of how EBV infects multiphenotypic lymphocytes and whether chronic active EBV infection is a truly monoclonal lymphoproliferative disease. We report two patients with chronic active EBV infection who showed expansion of multiphenotypic EBV-infected lymphocytes. EBV DNA was detected in CD4(+) and CD8(+) T cells and in B cells from pleural fluid of one patient and in T and B cells from a cervical lymph node of the other patient by polymerase chain reaction (PCR). Although real-time PCR showed that there were equally high loads of EBV genomes in CD4(+) and CD8(+) T cells from the pleural fluid, Southern blot hybridization with terminal repeats of the EBV genome showed a single band of the same molecular weight in three tissue samples from the patient. The results indicated biphenotypic expansions of CD4(+) and CD8(+) T cells infected with the same clone of EBV. Furthermore, bisulfite PCR analysis showed hypermethylated status in the Cp region in the two patients regardless of their cell populations. There has been a discrepancy between clonality and expansion of multiphenotypic EBV-infected lymphocytes. We speculate that lymphoid progenitor cells that have not differentiated into T and B cell progenitors are infected with EBV, resulting in clonal expansion of EBV-infected multiphenotypic cells. (C) 2004 Elsevier Ltd. All rights reserved.