Saliva of Lygus lineolaris digests double stranded ribonucleic acids
Saliva of Lygus lineolaris digests double stranded ribonucleic acids
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DOI：
10.1016/j.jinsphys.2011.12.014
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发表时间：
2012-03-01
影响因子：
2.2
通讯作者：
Walker, William B., III
中科院分区：
文献类型：
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作者：
Allen, Margaret L.;Walker, William B., III
基于双链核糖核酸的高特异性农药的开发前景是近年来科学研究的热点。创造性的应用已经提出并得到证明。然而，并不是所有的昆虫都对双链RNA（dsRNA）基因敲除效应敏感;例如，在鳞翅目中的应用已经取得了不同的成功。基因敲除已在半翅目的几个物种中得到证实。在我们的实验室中，敲除实验依赖于将dsRNA显微注射到失去光泽的植物昆虫Lygus lineolaris的血腔中。随后通过喂食将dsRNA递送至昆虫的实验在证明敲除方面反复失败，并且提出了dsRNA在与昆虫细胞接触之前被昆虫消化和降解的假设。将dsRNA暴露于昆虫唾液、昆虫唾液腺和昆虫血淋巴与商业RNAase III进行比较。L.发现lineolaris快速消化双链RNA。RNA酶抑制剂不影响酶活性，但热处理减慢酶活性。出版社：Elsevier Ltd
The prospects for development of highly specific pesticides based on double stranded ribonucleic acid have been a recent focus of scientific research. Creative applications have been proposed and demonstrated. However, not all insects are sensitive to double stranded RNA (dsRNA) gene knockdown effects; applications in the order Lepidoptera, for example, have met with varied success. Gene knockdown has been demonstrated in several species in the order Hemiptera. In our laboratory, knockdown experiments relied on microinjection of dsRNA into the hemocoel of the tarnished plant bug, Lygus lineolaris. Subsequent experiments delivering dsRNA to insects by feeding were repeatedly unsuccessful in demonstrating knockdown, and a hypothesis was formulated that the dsRNA was digested and degraded by the insect prior to contact with the insect cells. Exposure of dsRNA to insect saliva, insect salivary glands, and insect hemolymph was compared with commercial RNAase III. The saliva of L. lineolaris was found to rapidly digest double stranded RNA. RNAase inhibitor did not affect the activity but heat treatment slowed enzymatic activity. Published by Elsevier Ltd.