Testicular abnormalities in mice with Y chromosome deficiencies.
Testicular abnormalities in mice with Y chromosome deficiencies.
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Y染色体缺陷小鼠的睾丸异常。
DOI：
10.1095/biolreprod.116.144006
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发表时间：
2017
影响因子：
3.6
通讯作者：
Ward,MonikaA
中科院分区：
文献类型：
--
作者：
Ruthig,VictorA;Nielsen,Torbjoern;Riel,JonathanM;Yamauchi,Yasuhiro;Ortega,EgleA;Salvador,Quinci;Ward,MonikaA
我们最近研究了Y染色体基因只有两个、一个或没有Y染色体基因的小鼠在注射圆形精子细胞后产生单倍体圆形精子细胞和存活后代的能力。在这里，我们研究了这些小鼠生精管内生殖细胞和支持细胞以及睾丸间质组织的正常。我们在周期酸席夫和苏木精染色的小鼠睾丸切片上进行了精子发生和间质组织的定量分析。Y基因贡献有限的小鼠生精上皮存在多种细胞异常，其总数高于Y染色体完整的雄性小鼠。特定异常类型的分布在所测试的不同的基因类型中不同。Y基因受限的男性也有更多的睾丸巨噬细胞和内部血管结构。结果表明，小鼠Y染色体基因缺陷与生精上皮细胞异常和睾丸间质的某些改变有关。
We recently investigated mice with Y chromosome gene contribution limited to two, one, or no Y chromosome genes in respect to their ability to produce haploid round spermatids and live offspring following round spermatid injection. Here we explored the normalcy of germ cells and Sertoli cells within seminiferous tubules, and the interstitial tissue of the testis in these mice. We performed quantitative analysis of spermatogenesis and interstitial tissue on Periodic acid-Schiff and hematoxylin-stained mouse testis sections. The seminiferous epithelium of mice with limited Y gene contribution contained various cellular abnormalities, the total number of which was higher than in the males with an intact Y chromosome. The distribution of specific abnormality types varied among tested genotypes. The males with limited Y genes also had an increased population of testicular macrophages and internal vasculature structures. The data indicate that Y chromosome gene deficiencies in mice are associated with cellular abnormalities of the seminiferous epithelium and some changes within the testicular interstitium.