Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts
Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts
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DOI：
10.1038/sj.gt.3302687
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发表时间：
2006-04-01
期刊：
影响因子：
5.1
通讯作者：
George, A
中科院分区：
文献类型：
--
作者：
Almushayt, A;Narayanan, K;George, A
成牙细胞是从牙乳头分化出来的有丝分裂后细胞。这些细胞负责产生钙化的牙本质基质。髓质-成牙细胞间期包含未分化的间充质干细胞，这些干细胞在特定信号分子的作用下具有细胞分化为成牙细胞样细胞的能力。牙本质基质蛋白1 （DMP1）是一种参与矿化调控的牙本质非胶原细胞外基质蛋白。在这项研究中，我们在大鼠模型中研究了DMP1在诱导牙髓干细胞向成牙细胞样细胞分化和形成修复牙本质中的潜在作用。在上颌第一磨牙上钻牙洞，露出牙髓。1组直接植入重组DMP1浸渍的胶原基质，2组植入常用的盖髓剂氢氧化钙，3组直接植入未浸渍rDMP1的胶原基质作为对照。每组再分为两个亚组，A组2周时间，B组4周时间。实验结束后，切除上颌，对组织进行组织学和免疫组化评价。结果表明，DMP1对牙本质-牙髓复合体中未分化的间充质细胞具有形态形成作用。这些分化的细胞具有再生牙本质样组织的潜力，胶原基质、成牙本质特异性标记物和钙化沉积物的存在证实了这一点。
Odontoblasts are postmitotic cells that differentiate from the dental papilla. These cells are responsible for producing the calcified dentin matrix. The pulp-odontoblast interphase contains undifferentiated mesenchymal stem cells, which have the ability to cytodifferentiate into odontoblast-like cells in response to specific signaling molecules. Dentin matrix protein 1 (DMP1) is one of the dentin noncollagenous extracellular matrix proteins that has been implicated in regulation of mineralization. In this study, we have examined the potential role of DMP1 in inducing cytodifferentiation of dental pulp stem cells into odontoblast-like cells and formation of reparative dentin in a rat model. Cavities were drilled and pulps exposed in maxillary first molars. Collagen matrix impregnated with recombinant DMP1 was implanted directly in Group 1, while calcium hydroxide, a commonly used pulp-capping agent was implanted in group 2, collagen matrix that was not impregnated with rDMP1 was implanted directly in group 3, which served as control. Each of these three groups was subdivided into two subgroups, A for 2 weeks time duration and B for 4 weeks duration. At the end of the time period the maxillae were excised, tissues were processed for histological and immunohistochemical evaluations. The results showed that DMP1 could act as a morphogen on undifferentiated mesenchymal cells present in the dentin - pulp complex. These differentiated cells had the potential to regenerate dentin-like tissue, which was confirmed by the presence of collagenous matrix, odontoblast specific markers and calcified deposits.