Biodentine Boosts, WhiteProRoot®MTA Increases and Life® Suppresses Odontoblast Activity
Biodentine Boosts, WhiteProRoot®MTA Increases and Life® Suppresses Odontoblast Activity
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DOI：
10.3390/ma12071184
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发表时间：
2019-04-10
期刊：
影响因子：
3.4
通讯作者：
Carrilho, Eunice
中科院分区：
文献类型：
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作者：
Paula, Anabela;Laranjo, Mafalda;Carrilho, Eunice
(1)背景：当牙髓暴露时，可通过直接盖髓的方法诱导修复性牙本质形成，以维持组织的活力和功能。本工作的目的是评估三种不同的直接盖髓材料，氢氧化钙(LIFE((R)，矿物三氧化物聚集体(WhiteProRoot((R))MTA)和硅酸钙(Biodentine)在成牙本质细胞样鼠细胞系(MDPC-23)中的细胞毒性和生物活性。(2)用四甲基偶氮唑蓝(3-(4，5-dimethylthiazol-2-yl)-2，5-diphenyltetrazolium)比色法测定细胞代谢活性，用硫代罗丹明B比色法测定细胞存活率，用流式细胞仪检测细胞死亡类型和细胞周期。聚合酶链式反应检测碱性磷酸酶活性，免疫细胞化学法检测牙本质唾液蛋白的表达。矿化采用茜素红S比色法测定，分光光度法定量。(3)结果：LIFE((R))引起细胞代谢活性和活性下降，并与细胞死亡增加有关。在细胞毒性试验中，WhiteProRoot((R))MTA和Biodentine诱导了类似的效应，牙本质唾液蛋白(DSP)的表达增加，矿化沉积物的形成，尤其是Biodentine。(4)结论：WhiteProRoot((R))MTA的结果证实了它在这些疗法中的适应症，证明它是公认的黄金标准。生物牙本质可能是另一种选择，因为它们促进的细胞反应与三氧化二矿聚集体(MTA)相同。
(1) Background: When pulp exposure occurs, reparative dentinogenesis can be induced by direct pulp capping to maintain the vitality and function of the tissue. The aim of this work was to assess the cytotoxicity and bioactivity of three different direct pulp capping materials, calcium hydroxide (Life((R))), mineral trioxide aggregate (WhiteProRoot((R))MTA) and calcium silicate (Biodentine), in an odontoblast-like mouse cell line (MDPC-23). (2) Methods: Metabolic activity was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test (MTT)assay, viability by the sulforhodamine B (SRB) assay, and the type of death and cell cycle analysis by flow cytometry. Alkaline phosphatase was evaluated by polymerase chain reaction (PCR), and dentin sialoprotein expression was assessed by immunocytochemistry. Mineralization was determined by the Alizarin Red S colorimetric assay and quantified by spectrophotometry. (3) Results: Life((R)) induced a decrease in metabolic activity and viability, which is associated with an increase cell death. WhiteProRoot((R))MTA and Biodentine induced similar effects in cytotoxicity assays, with an increase in the expression of dentin sialoprotein (DSP) and formation of mineralized deposits, especially with Biodentine. (4) Conclusions: The results of WhiteProRoot((R))MTA confirm its indication for these therapies, justifying its recognition as the gold standard. Biodentine may be an alternative, since they promote the same cellular response that mineral trioxide aggregate (MTA) does.