Development of capping ability during differentiation of HL-60 human promyelocytic leukemia cells.
Development of capping ability during differentiation of HL-60 human promyelocytic leukemia cells.
复制标题
HL-60 人早幼粒细胞白血病细胞分化过程中加帽能力的发展。
DOI：
10.1002/jcp.1041060114
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发表时间：
1981
影响因子：
5.6
通讯作者：
Snell,WJ
中科院分区：
文献类型：
--
作者：
Brown,WJ;Norwood,CF;Smith,RG;Snell,WJ
在人早幼粒细胞白血病细胞（HL-60系）中研究了细胞表面和细胞骨架元素的发育变化，该细胞在添加二甲基亚砜（DMSO）的培养基中生长时分化为功能成熟的髓样细胞。分化和未分化的HL-60细胞均在整个细胞表面以弥漫模式结合荧光伴刀豆球蛋白A（F-Con A）。与正常中性粒细胞一样，在与ConA孵育之前，用秋水仙碱预处理分化的HL-60细胞，导致形成大的胞质突起，凝集素在突起上结合形成帽。另一方面，类似处理的未分化HL-60细胞不形成细胞质突起，并且不能覆盖Con A。透射电子显微镜显示，在分化过程中，微管和微丝的数量和分布发生变化。因此，发育中的骨髓细胞在分化为成熟吞噬细胞时，其细胞骨架的结构和功能发生了重要的变化。
Developmental changes in cell surface and cytoskeletal elements have been studied in human promyelocytic leukemia cells (line HL‐60) which differentiate into functionally mature myeloid cells when grown in dimethyl sulfoxide (DMSO)‐supplemented medium. Both differentiated and undifferentiated HL‐60 cells bind fluorescent concanavalin A (F‐Con A) in a diffuse pattern over the entire cell surface. As with normal neutrophils, pretreatment of thedifferentiatedHL‐60 cells with colchicine before incubation with Con A causes the formation of large cytoplasmic protrusions over which the lectin associates into a cap. On the other hand, similarly treated undifferentiated HL‐60 cells do not form the cytoplasmic protuberances and are unable to cap the Con A. Transmission electron microscopy reveals that the number and distribution of microtubules and microfilaments change during differentiation. Thus, developing myeloid cells undergo important alterations in the structure and function of the cytoskeleton as they differentiate into mature phagocytes.