Identification of novel genes coding for small expressed RNAs
Identification of novel genes coding for small expressed RNAs
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DOI：
10.1126/science.1064921
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发表时间：
2001-10-26
期刊：
影响因子：
56.9
通讯作者：
Tuschl, T
中科院分区：
文献类型：
--
作者：
Lagos-Quintana, M;Rauhut, R;Tuschl, T
在秀丽隐杆线虫中，LIN-4和Let-7分别编码22-和21-核苷酸（NT）RNA，它们是发育时机的关键调节剂。由于这些短RNA的外观在开发过程中受到调节，因此它们也称为小时RNA（strnas）。我们表明，许多21 nt和22-nt表达的RNA，称为microRNA，存在于无脊椎动物和脊椎动物中，并且其中一些新型RNA（类似于let-7 strna）是高度保守的。这表明由小RNA介导的序列特异性的，转录后调节机制比以前所欣赏的要笼统。
In Caenorhabditis elegans, lin-4 and let-7 encode 22- and 21-nucleotide (nt) RNAs, respectively, which function as key regulators of developmental timing. Because the appearance of these short RNAs is regulated during development, they are also referred to as small temporal RNAs (stRNAs). We show that many 21- and 22-nt expressed RNAs, termed microRNAs, exist in invertebrates and vertebrates and that some of these novel RNAs, similar to let-7 stRNA, are highly conserved. This suggests that sequence-specific, posttranscriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated.