Biomarker discovery in asthma-related inflammation and remodeling
Biomarker discovery in asthma-related inflammation and remodeling
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DOI：
10.1002/pmic.200800643
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发表时间：
2009-04-01
期刊：
影响因子：
3.4
通讯作者：
Cataldo, Didier
中科院分区：
文献类型：
--
作者：
Calvo, Florence Quesada;Fillet, Marianne;Cataldo, Didier
哮喘是一种复杂的气道炎症性疾病。炎症细胞、肽介质、细胞外基质成分和蛋白酶之间相互作用的网络被认为参与了哮喘相关气道炎症和重塑的建立和维持。迄今为止，在哮喘病理生理中显示重要活性的新蛋白质介质仍有待发现。本研究的主要目的是通过使用表面增强激光解吸/电离飞行时间质谱（SELDI-TOF-MS）对来自过敏原诱导的气道炎症和重塑模型的小鼠肺样本进行分析，以发现潜在的靶蛋白。在这个模型中，我们指出了几个蛋白或肽峰，与对照组相比，这些蛋白或肽峰在患病小鼠中优先表达。我们报告了不同的五种蛋白的鉴定：发现炎性区I或RELM α (FIZZ-1)，钙调素（S100A6）， clara细胞分泌蛋白10 (CC10)，泛素和组蛋白H4。
Asthma is a complex inflammatory disease of airways. A network of reciprocal interactions between inflammatory cells, peptidic mediators, extracellular matrix components, and proteases is thought to be involved in the installation and maintenance of asthma-related airway inflammation and remodeling. To date, new proteic mediators displaying significant activity in the pathophysiology of asthma are still to be unveiled. The main objective of this study was to uncover potential target proteins by using surface-enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) on lung samples from mouse models of allergen-induced airway inflammation and remodeling. In this model, we pointed out several protein or peptide peaks that were preferentially expressed in diseased mice as compared to controls. We report the identification of different five proteins: found inflammatory zone I or RELM alpha (FIZZ-1), calcyclin (S100A6), clara cell secretory protein 10 (CC10), Ubiquitin, and Histone H4.