Probing cellular traction forces with magnetic nanowires and microfabricated force sensor arrays.
Probing cellular traction forces with magnetic nanowires and microfabricated force sensor arrays.
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DOI：
10.1088/0957-4484/23/7/075101
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发表时间：
2012-02-24
期刊：
影响因子：
3.5
通讯作者：
Reich DH
中科院分区：
文献类型：
--
作者：
Lin YC;Kramer CM;Chen CS;Reich DH
在本文中，使用磁性纳米线的细胞响应力的研究证明。将直径300 nm、长度达20 μm的高纵横比镍棒与培养在柔性微柱力传感器阵列上的肺动脉平滑肌细胞（SMC）结合或内化。通过用频率范围为0.1 - 10 Hz的AC磁场驱动纳米线向细胞施加力和扭矩，并用微柱记录细胞收缩力的变化。这些局部刺激会产生细胞牵引力的整体力增强，但添加钙通道阻滞剂钌红后，这种收缩增强可以有效抑制，这表明钙通道在机械反应中的作用。SMC对致动的响应性取决于所施加刺激的频率。这些结果表明，磁性纳米颗粒和微图案化的柔性基底的组合可以为细胞力学转导的研究提供新的途径。
In this paper, the use of magnetic nanowires for the study of cellular response to force is demonstrated. High-aspect ratio Ni rods with diameter 300 nm and lengths up to 20 μm were bound to or internalized by pulmonary artery smooth muscle cells (SMCs) cultured on arrays of flexible micropost force sensors. Forces and torques were applied to the cells by driving the nanowires with AC magnetic fields in the frequency range 0.1 – 10 Hz, and the changes in cellular contractile forces were recorded with the microposts. These local stimulations yield global force reinforcement of the cells’ traction forces, but this contractile reinforcement can be effectively suppressed upon addition of a calcium channel blocker, ruthenium red, suggesting the role of calcium channels in the mechanical response. The responsiveness of the SMCs to actuation depends on the frequency of the applied stimulation. These results show that the combination of magnetic nanoparticles and micropatterned, flexible substrates can provide new approaches to the study of cellular mechanotransduction.