TRANSCRIPTIONAL REPRESSOR ZF5 IDENTIFIES A NEW CONSERVED DOMAIN IN ZINC-FINGER PROTEINS
TRANSCRIPTIONAL REPRESSOR ZF5 IDENTIFIES A NEW CONSERVED DOMAIN IN ZINC-FINGER PROTEINS
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DOI：
10.1093/nar/21.16.3767
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发表时间：
1993-08-11
影响因子：
14.9
通讯作者：
CALAME, K
中科院分区：
文献类型：
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作者：
NUMOTO, M;NIWA, O;CALAME, K
我们克隆了一种新的小鼠C2H2锌指蛋白ZF5的编码基因。51.3kD的蛋白质在C末端含有5个GL1-Kruppel类型的锌指。在其N-末端，ZF5有一个41个氨基酸的区域，被发现与其他几个锌指蛋白的N-末端同源。这个区域在锌指蛋白中定义了一个新的基序，我们称之为锌指N-末端(Zin)结构域。ZF5结合在c-myc启动子的两个位置和单纯疱疹病毒胸苷激酶启动子的-50个碱基上。ZF5是一个转录抑制因子，其抑制域位于锌指结构域的N端。一个4kb的ZF5基因被广泛表达。
We have cloned a cDNA encoding a new murine C2H2 zinc finger protein, ZF5. The 51.3 kD protein contains five GL1-Kruppel type zinc fingers at the C-terminus. At its N-terminus, ZF5 has a 41 amino acid region which was found to be homologous to the N-termini of several other zinc finger proteins. This region defines a new motif within zinc finger proteins which we have named the Zinc finger N-terminal (ZiN) domain.ZF5 binds to two sites in the c-myc promoter and to the -50 bp site of the herpes simplex thymidine kinase promoter. ZF5 is a transcriptional repressor and its repression domain is located N-terminal to the zinc finger domains. A single 4 kb ZF5 mRNA is expressed widely.