Selective recovery of founder genetic diversity in aquacultural broodstocks and captive, endangered fish populations
Selective recovery of founder genetic diversity in aquacultural broodstocks and captive, endangered fish populations
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选择性恢复水产亲鱼和圈养濒危鱼类种群的创始人遗传多样性
DOI：
10.1023/a:1013772205330
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发表时间：
2004
期刊：
影响因子：
1.5
通讯作者：
N. Taniguchi
中科院分区：
文献类型：
--
作者：
R. Doyle;R. Pérez;M. Takagi;N. Taniguchi
用于遗传保护或水产养殖的孵化场种鱼可能不能很好地代表它们祖先的基因库。当发现亲鱼的鱼是彼此的近亲时，这种情况尤其可能发生。我们重新分析了红海鲷的繁殖实验的微卫星数据，以证明当基因频率被孵化场的近亲创始人扭曲时，丢失的遗传变异是如何恢复的。使用基于亲缘关系估计器的最小亲属标准来选择代表最大创始谱系数量（即携带祖先基因相同拷贝数最少）的育种者子集。Nei遗传距离的UPGMA聚类将这些选定的亚群与亲代基因库分组，而不是与整个高度“漂移”的后代分组。所选择的亚群也捕获了许多亲本基因库的预期杂合性和等位基因多样性。同一条鱼的独立系谱数据表明，选择的子集比相同大小的随机子集有更多的贡献父母和更多的创始人等同物。在选定的子集中，估计的平均共祖较低，这意味着如果他们被用作育种者，后代的近亲繁殖将较低。该程序似乎适合于减少由于近亲和孵化场基因库中过多代表的创始人造成的遗传扭曲。
Hatchery broodstocks used for genetic conservation or aquaculture may represent their ancestral gene pools rather poorly. This is especially likely when the fish that found a broodstock are close relatives of each other. We re-analysed microsatellite data from a breeding experiment on red sea bream to demonstrate how lost genetic variation might be recovered when gene frequencies have been distorted by consanguineous founders in a hatchery. A minimal-kinship criterion based on a relatedness estimator was used to select subsets of breeders which represented the maximum number of founder lineages (i.e., carried the fewest identical copies of ancestral genes). UPGMA clustering of Nei's genetic distances grouped these selected subsets with the parental gene pool, rather than with the entire, highly ‘drifted’ offspring generation. The selected subsets also captured much of the expected heterozygosity and allelic diversity of the parental gene pool. Independent pedigree data on the same fish showed that the selected subsets had more contributing parents and more founder equivalents than random subsets of the same size. The estimated mean coancestry was lower in the selected subsets, meaning that inbreeding in subsequent generations would be lower if they were used as breeders. The procedure appears suitable for reducing the genetic distortion due to consanguineous and over-represented founders of a hatchery gene pool.
近亲繁殖：一个词，几个意思，很混乱。
DOI：
10.1007/978-3-0348-8510-2_9
发表时间：
1994
期刊：
EXS
影响因子：
--
作者：
Templeton,AR;Read,B
通讯作者：
Read,B