Comparing the immunosuppressive potency of naïve marrow stromal cells and Notch-transfected marrow stromal cells.
Comparing the immunosuppressive potency of naïve marrow stromal cells and Notch-transfected marrow stromal cells.
复制标题
DOI：
10.1186/1742-2094-8-133
复制
发表时间：
2011-10-07
影响因子：
9.3
通讯作者：
Case CC
中科院分区：
文献类型：
--
作者：
Dao MA;Tate CC;Aizman I;McGrogan M;Case CC
从用Notch胞内结构域（NICD）表达质粒转染的骨髓基质细胞（MSC）扩增SB 623细胞。在中风诱导的动物中，这些细胞减少梗死面积并促进功能恢复。SB 623细胞在形态学和细胞表面标志物方面类似于亲本MSC，尽管已经在延长的培养中。已知MSC具有免疫抑制特性; MSC的长期培养是否影响其免疫调节活性尚未得到解决。为了评估SB 623细胞可能的衰老特性，我们进行了细胞周期相关测定和β-半乳糖苷酶染色。为了评估这些扩增的NICD转染的MSC的免疫调节活性，我们进行了SB 623细胞或MSC与富集的人T细胞或单核细胞的共培养，并通过流式细胞术评估了细胞因子的产生。此外，我们监测了SB 623细胞在同种异体和异种混合淋巴细胞反应（MLR）中的免疫抑制活性。与MSC相比，我们发现在每批SB 623细胞中出现少量衰老样细胞。然而，我们证明，这些细胞抑制人类T细胞增殖的同种异体和异种混合淋巴细胞反应（MLR）的方式与MSC。产生产生IL-10的T细胞，并且通过与SB 623细胞共培养抑制单核细胞-树突状细胞分化。与亲本MSC相比，SB 623细胞似乎对树突状细胞的成熟产生更大的抑制作用，如通过共刺激分子CD 86的表面表达的更大降低所证明的。结果表明，扩增的NICD转染的MSC的免疫抑制活性与亲本MSC相当，尽管出现少量的衰老样细胞。
SB623 cells are expanded from marrow stromal cells (MSCs) transfected with a Notch intracellular domain (NICD)-expressing plasmid. In stroke-induced animals, these cells reduce infarct size and promote functional recovery. SB623 cells resemble the parental MSCs with respect to morphology and cell surface markers despite having been in extended culture. MSCs are known to have immunosuppressive properties; whether long-term culture of MSCs impact their immunomodulatory activity has not been addressed. To assess the possible senescent properties of SB623 cells, we performed cell cycle related assays and beta-galactosidase staining. To assess the immunomodulatory activity of these expanded NICD-transfected MSCs, we performed co-cultures of SB623 cells or MSCs with either enriched human T cells or monocytes and assessed cytokine production by flow cytometry. In addition, we monitored the immunosuppressive activity of SB623 cells in both allogenic and xenogenic mixed lymphocyte reaction (MLR). Compared to MSCs, we showed that a small number of senescent-like cells appear in each lot of SB623 cells. Nevertheless, we demonstrated that these cells suppress human T cell proliferation in both the allogeneic and xenogeneic mixed lymphocyte reaction (MLR) in a manner comparable to MSCs. IL-10 producing T cells were generated and monocyte-dendritic cell differentiation was dampened by co-culture with SB623 cells. Compared to the parental MSCs, SB623 cells appear to exert a greater inhibitory impact on the maturation of dendritic cells as demonstrated by a greater reduction in the surface expression of the co-stimulatory molecule, CD86. The results demonstrated that the immunosuppressive activity of the expanded NICD-transfected MSCs is comparable to the parental MSCs, in spite of the appearance of a small number of senescent-like cells.
登录
查看更多内容
DOI：
10.1084/jem.193.11.1303
发表时间：
2001-06-04
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Dieckmann D;Plottner H;Berchtold S;Berger T;Schuler G
通讯作者：
Schuler G
影响因子：
13.6
作者：
Freund A;Orjalo AV;Desprez PY;Campisi J
通讯作者：
Campisi J
影响因子：
5.2
作者：
Djouad, Farida;Charbonnier, Louis-Marie;Noel, Daniele
通讯作者：
Noel, Daniele
影响因子：
4.4
作者：
Asano, Naoki;Watanabe, Tomohiro;Strober, Warren
通讯作者：
Strober, Warren
影响因子：
4.3
作者：
Ou-Yang, Hai-Feng;Zhang, Hong-Wei;Han, Hua
通讯作者：
Han, Hua