Biological roles of dermatan sulphate proteoglycans.
Biological roles of dermatan sulphate proteoglycans.
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硫酸皮肤素蛋白聚糖的生物学作用。
DOI：
10.1002/9780470513385.ch4
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Culp,LA
中科院分区：
文献类型：
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作者：
Rosenberg,LC;Choi,HU;Poole,AR;Lewandowska,K;Culp,LA
含有皮肤硫酸盐的蛋白聚糖（DS - pg）广泛分布于皮肤、巩膜、肌腱、软骨和各种其他结缔组织的细胞外基质中。最近从成熟的牛关节软骨中分离出两种皮肤硫酸蛋白多糖，分别称为DS - PGI和DS - PGII。在它们的单体形式中，DS - PGI和DS - PGII都是多分散的，相对分子质量（Mr）范围在80K到140K之间，并且具有明显Mr值约为45K的蛋白核。DS - PGI容易自我关联，而DS - PGII则不容易。抗DS - PGII的多克隆和单克隆抗体不与DS - PGI发生反应。DS - PGI和DS - PGII似乎具有不同的核心蛋白，代表了两种不同的皮肤硫酸盐蛋白多糖。DS‐pg对细胞的生物学功能有显著影响。例如，它们抑制成纤维细胞粘附纤维连接蛋白基质的能力。用[3H]胸苷标记BALB/c 3T3细胞，并将其镀在涂有血浆纤维连接蛋白、血浆纤维连接蛋白加硫酸软骨素蛋白多糖（CS - PG，软骨特异性蛋白多糖单体）或血浆纤维连接蛋白加DS - PG的培养皿上。在不含蛋白多糖的情况下，约55%的细胞在1小时内附着。在CS - PG存在的情况下，细胞附着略有下降。在DS‐pg存在的情况下，成纤维细胞与纤维连接蛋白的粘附基本被消除。如果在血浆纤维连接蛋白基质上预吸附DS - pg，并将DS - pg留在附着介质中，则可以获得类似的结果。
Dermatan sulphate‐containing proteoglycans (DS‐PGs) are widely distributed in the extracellular matrix of skin, sclera, tendon, cartilage and a variety of other connective tissues. Two species of dermatan sulphate proteoglycans, called DS‐PGI and DS‐PGII, have recently been isolated from mature bovine articular cartilages. In their monomeric forms, both DS‐PGI and DS‐PGII are polydisperse, have relative molecular masses (Mr) ranging from 80K to 140K, and possess protein cores with apparentMrvalues of approximately 45K. DS‐PGI readily self‐associates whereas DS‐PGII does not. Polyclonal and monoclonal antibodies against DS‐PGII do not react with DS‐PGI. DS‐PGI and DS‐PGII appear to possess different core proteins and represent two different species of dermatan sulphate proteoglycans.DS‐PGs have dramatic effects on the biological functions of cells. For example, they inhibit the capacity of fibroblasts to adhere to a fibronectin substratum. BALB/c 3T3 cells were labelled with [3H]thymidine and plated onto dishes coated with plasma fibronectin, plasma fibronectin plus chondroitin sulphate proteoglycan (CS‐PG, cartilage‐specific proteoglycan monomer), or plasma fibronectin plus DS‐PGs. In the absence of proteoglycan, approximately 55% of the cells were attached at 1 h. In the presence of CS‐PG, cell attachment was slightly decreased. In the presence of DS‐PGs, the adhesion of the fibroblasts to fibronectin was essentially abolished. Similar results were obtained if a plasma fibronectin substratum was preadsorbed with the DS‐PGs and the DS‐PGs were left in the attachment medium.